https://scholars.lib.ntu.edu.tw/handle/123456789/524037
標題: | Sialomucin expression is associated with erbB-2 oncoprotein overexpression, early recurrence, and cancer death in non-small-cell lung cancer | 作者: | CHONG-JEN YU CHIA-TUNG SHUN PAN-CHYR YANG Lee Y.-C. Shew J.-Y. Kuo S.-H. KWEN-TAY LUH |
公開日期: | 1997 | 出版社: | American Thoracic Society | 卷: | 155 | 期: | 4 | 起(迄)頁: | 1419-1427 | 來源出版物: | American Journal of Respiratory and Critical Care Medicine | 摘要: | Mucin production, when heavily sialylated, can promote cancer cell invasion and metastasis, and modulate the immune recognition system of the host. To explore the prognostic implication of sialomucin expression in lung cancer, we studied 116 patients with non-small-cell lung cancer (NSCLC). Tumor specimens were stained immunohistochemically with monoclonal antibodies (mAbs) against mucin glycoprotein (17Q2, HMFG2, SM3), and histochemically with periodic acid-Schiff/alcian blue to differentiate neutral mucin from acid mucin, and with high-iron diamine/alcian blue to differentiate sialomucin from sulfomucin. The expression status of two established molecular prognostic factors, the p53 and erbB-2 oncoproteins, were evaluated immunohistochemically. The staining was performed on two separately archived, paraffin-embedded tumor blocks for each patient, with normal lung as a control. Correlations were subsequently made among stains and various clinicopathologic factors. All analyses were blinded, and included Kaplan- Meier survival estimates with Cox proportional hazards regression modeling. Associations were established among adenocarcinoma histotype and erbB-2 overexpression, sialomucin expression, and 17Q2 and HMFG2 immunohistochemical positivity (p < 0.05). Sialomucin expression was closely linked to erbB-2 overexpression (p = 0.01). Significant univariate predictors (p < 0.05) of recurrence and cancer death were surgical stage, p53 expression, erbB-2 overexpression, and sialomucin expression. These four factors remained as independent predictors of early recurrence (p < 0.05) after multivariate analysis. For cancer death prediction, p53 and sialomucin expression had a marginal effect. We concluded that sialomucin expression is also a poor indicator of prognosis, which is associated with erbB-2 oncoprotein overexpression, early postoperative recurrence, and cancer death in NSCLC. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030935043&doi=10.1164%2fajrccm.155.4.9105088&partnerID=40&md5=8180d3be7e910a617b185d1892699530 https://scholars.lib.ntu.edu.tw/handle/123456789/524037 |
ISSN: | 1073-449X | DOI: | 10.1164/ajrccm.155.4.9105088 | SDG/關鍵字: | alcian blue; diamine; iron; monoclonal antibody; mucin; oncoprotein; periodic acid; protein p53; sialomucin; adult; aged; article; cancer mortality; cancer recurrence; female; human; human tissue; immunohistochemistry; lung non small cell cancer; major clinical study; male; priority journal |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。