Molecular detection and incidence of human papillomavirus in neonates: Methodology and a pilot study in a medical center
Journal
Journal of Microbiology, Immunology and Infection
Journal Volume
45
Journal Issue
3
Pages
185-192
Date Issued
2012
Author(s)
Tai C.-F.
Tsou T.-P.
Hsieh W.-S.
Hsu C.-H.
Liau Y.-J.
Abstract
Background/Purpose: Human papillomavirus (HPV) infection can cause laryngeal papillomas in children. Vertical transmission has been confirmed. This study aimed to establish a sensitive molecular diagnostic method and understand the incidence of the HPV-6 and HPV-11 in neonates with intubation. Methods: We enrolled 108 newborns between October 2007 and January 2010. All neonates were intubated due to underlying disease. The specimens were collected via endotracheal aspiration. DNA of HPV types 6 and 11 was detected by real-time PCR and nested PCR. Results: HPV-DNA was detected in eight of the 108 newborns studied. Seven respiratory specimens tested positive for HPV-11 and one was positive for HPV-6. The HPV 6/11 detection rate in neonates was 7.4% (8/108). Conclusion: A rapid, sensitive, specific, and reproducible RT-PCR method and nest PCR were developed for the detection and differentiation of HPV-6 and HPV-11 genomic variants in a single PCR reaction. The assays are of great value for clinical and epidemiologic studies of HPV-6 and HPV-11 infections. Neonatal HPV colonization may be related to juvenile-onset recurrent respiratory papillomatosis. The transmission route may be from mother to child. The clinical significance of neonatal carriage of HPV-6 or HPV-11 warrants further study. ? 2012 .
SDGs
Other Subjects
virus DNA; article; genetic variability; human; Human papillomavirus type 11; Human papillomavirus type 6; incidence; intubation; major clinical study; molecular diagnosis; newborn; pilot study; real time polymerase chain reaction; reproducibility; respiratory system; sensitivity and specificity; tracheal aspiration procedure; virus transmission; Academic Medical Centers; Base Sequence; Cohort Studies; DNA, Viral; Human papillomavirus 11; Human papillomavirus 6; Humans; Incidence; Infant, Newborn; Intubation, Intratracheal; Molecular Diagnostic Techniques; Molecular Sequence Data; Papillomavirus Infections; Pilot Projects; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Alignment
Type
journal article