Skin-homing CD4+ Foxp3+ T cells exert Th2-like function after staphylococcal superantigen stimulation in atopic dermatitis patients
Journal
Clinical and Experimental Allergy
Journal Volume
41
Journal Issue
4
Pages
516-525
Date Issued
2011
Author(s)
Abstract
Background The effect of staphylococcal superantigens (SsAgs) on cutaneous lymphocyte-associated antigen (CLA)+ CD4+ Foxp3+ T cells of atopic dermatitis (AD) patients is unknown. Objective To compare the effects of SsAgs on the ratio, function, and apoptosis of CCR6+ subtype and CCR6- subtype of CLA+ CD4+ Foxp3+ T cells among AD patients, asthma/allergic rhinitis (AR) patients without AD, and healthy subjects. Methods Using immunofluorescence staining followed by flow cytometric analysis, we analysed peripheral blood mononuclear cells cultured with or without staphylococcal enterotoxin B (SEB) stimulation in 20 AD patients, 20 asthma/AR patients without AD, and 20 healthy subjects. Results SEB decreased CCR6+/CCR6- ratio in CLA+ CD4+ Foxp3+ T cells from AD patients and increased CCR6+/CCR6- ratio in those from healthy subjects. SEB induced the production of type 2 T helper cell (Th2) cytokine interleukin (IL)-5 in CCR6- subtype and anti-inflammatory cytokine IL-10 in CCR6+ subtype of CLA+ CD4+ Foxp3+ T cells. CLA+ CD4+ Foxp3+ T cells from AD patients produced more IL-5 and less IL-10 after SEB stimulation than those from healthy subjects. CCR6- subtype of CLA+ CD4+ Foxp3+ T cells from AD patients and CCR6+ subtype of those cells from healthy subjects were more resistant to SEB-induced caspase-3 activation than the other subtype and those from other subjects. Conclusions and Clinical Relevance Despite a phenotype of regulatory T cells, skin-homing CD4+ Foxp3+ T cells of AD patients exert effector Th2-like function after SsAgs stimulation, which may aggravate allergic skin inflammation. ? 2011 Blackwell Publishing Ltd.
SDGs
Other Subjects
bacterial antigen; caspase 3; CD4 antigen; chemokine receptor CCR6; cutaneous lymphocyte associated antigen; interleukin 10 antibody; interleukin 5; staphylococcal superantigen; Staphylococcus enterotoxin B; transcription factor FOXP3; unclassified drug; allergic rhinitis; apoptosis; article; asthma; atopic dermatitis; cell culture; cell function; clinical article; controlled study; cross-sectional study; cytokine release; enzyme activation; female; flow cytometry; human; immunofluorescence; immunostimulation; lymphocyte homing; lymphocyte proliferation; male; peripheral blood mononuclear cell; priority journal; T lymphocyte; Th2 cell; Adolescent; Antigens, Differentiation, T-Lymphocyte; Cell Separation; Cross-Sectional Studies; Cytokines; Dermatitis, Atopic; Enterotoxins; Female; Flow Cytometry; Fluorescent Antibody Technique; Forkhead Transcription Factors; Humans; Male; Membrane Glycoproteins; Skin; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Th2 Cells
Type
journal article