https://scholars.lib.ntu.edu.tw/handle/123456789/527136
標題: | Single-Cell-Derived Tumor-Sphere Formation and Drug-Resistance Assay Using an Integrated Microfluidics | 作者: | Pang, L. Ding, J. Ge, Y. Fan, J. Fan, S.-K. SHIH-KANG FAN |
公開日期: | 2019 | 卷: | 91 | 期: | 13 | 起(迄)頁: | 8318-8325 | 來源出版物: | Analytical Chemistry | 摘要: | Considerable evidence points to cancer stem-like cells (CSCs) as responsible for promoting progression, metastasis, and drug resistance. Without damage to the cell biological properties, single-cell-derived tumor-sphere is encouraging options for CSCs identification and studies. Although several single cell-based microfluidic methods have been developed for CSCs studies, clarifying liaison between the biomechanics of cells (such as size and deformability) and stem (such as tumor-sphere formation and drug resistance) remains challenging. Herein, we present a platform of integrated microfluidics for the analysis of single-cell-derived tumor-sphere formation and drug resistance. Tumor-spheres derived from different biomechanics (size and/or deformation) single-cells could be formed efficiently using this device. To demonstrate the microfluidic-platform capability, a proof-of-concept experiment was implemented by evaluating single-cell-derived sphere formation of single glioblastoma cells with different biomechanics. Additionally, a course of chemotherapy to study these single-cell-derived spheres was determined by coculture with vincristine. The results indicate that tumor cell biomechanics is associated with single-cell-derived spheres formation; that is, smaller and/or more deformable tumor cells are more stem-like defined by the formation of single-cell-derived spheres than more prominent and/or lesser deformable tumor cells. Also, tumor-spheres derived from single small and/or more deformable tumor cell have higher drug resistance than more prominent and/or less deformable tumor cells. Our device offers a new approach for single-cell-derived sphere formation according to tumor cell different biomechanical properties. Furthermore, it offers a new method for CSC identification and downstream analysis on a single-cell level. ? 2019 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85067953379&partnerID=40&md5=d3c55ff52023be462907feee0cb7cec3 https://scholars.lib.ntu.edu.tw/handle/123456789/527136 |
DOI: | 10.1021/acs.analchem.9b01084 | SDG/關鍵字: | Biomechanics; Biophysics; Cells; Chemotherapy; Damage detection; Deformation; Microfluidics; Spheres; Tumors; Biological properties; Biomechanical properties; Glioblastoma cells; Integrated microfluidics; Microfluidic method; Microfluidic platforms; Proof of concept; Single-cell level; Cytology |
顯示於: | 機械工程學系 |
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