https://scholars.lib.ntu.edu.tw/handle/123456789/528149
標題: | In vitro diagnostics of coronavirus disease 2019: Technologies and application | 作者: | Lai C.-C. Wang C.-Y. Ko W.-C. PO-REN HSUEH |
關鍵字: | Anti-SARS-CoV-2 antibody; Coronavirus disease-2019; Lateral flow immunoassay; Point-of-care; Real-time reverse transcription PCR | 公開日期: | 2020 | 來源出版物: | Journal of Microbiology, Immunology and Infection | 摘要: | Laboratory-based diagnostic measures including virological and serological tests are essential for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time reverse transcription-polymerase chain reactions (rRT-PCR) can detect SARS-COV-2 by targeting open reading frame-1 antibodies (ORF1ab), envelope protein, nucleocapsid protein, RNA-dependent RNA polymerase genes, and the N1, N2, and N3 (3N) target genes. Therefore, rRT-PCR remains the primary method of diagnosing SARS-CoV-2 despite being limited by false-negative results, long turnaround, complex protocols, and a need for skilled personnel. Serological diagnosis of coronavirus disease 2019 (COVID-19) is simple and does not require complex techniques and equipment, rendering it suitable for rapid detection and massive screening. However, serological tests cannot confirm SARS-CoV-2, and results will be false-negative when antibody concentrations fall below detection limits. Balancing the increased use of laboratory tests, risk of testing errors, need for tests, burden on healthcare systems, benefits of early diagnosis, and risk of unnecessary exposure is a significant and persistent challenge in diagnosing COVID-19. ? 2021 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/528149 | ISSN: | 1684-1182 | DOI: | 10.1016/j.jmii.2020.05.016 | SDG/關鍵字: | envelope protein; nucleocapsid protein; RNA directed RNA polymerase; antibody blood level; coronavirus disease 2019; diagnostic procedure; early diagnosis; false negative result; health care system; human; laboratory test; multiplex polymerase chain reaction; open reading frame; real time reverse transcription polymerase chain reaction; Review; serology; diagnosis; false positive result; genetics; immunology; in vitro study; isolation and purification; pandemic; procedures; sensitivity and specificity; virology; COVID-19; COVID-19 Nucleic Acid Testing; COVID-19 Serological Testing; COVID-19 Testing; False Negative Reactions; False Positive Reactions; Humans; In Vitro Techniques; Pandemics; SARS-CoV-2; Sensitivity and Specificity |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。