https://scholars.lib.ntu.edu.tw/handle/123456789/528672
Title: | Rapid identification of the Mycobacterium tuberculosis complex by an enzyme-linked immunosorbent assay | Authors: | Chou C.-H. YU-TSUNG HUANG Hsu H.-L. Lai C.-C. Liao C.-H. PO-REN HSUEH |
Issue Date: | 2009 | Journal Volume: | 13 | Journal Issue: | 8 | Start page/Pages: | 996-1001 | Source: | International Journal of Tuberculosis and Lung Disease | Abstract: | SETTlNG: A medical centre in Taipei, Taiwan. OBJECTIVE: To investigate the performance of an enzyme-linked immunosorbent assay (ELISA) using anti-early secreted antigenic target 6 and culture filtrate protein 10 antibodies (MeDiPro Mycobacterium tuberculosis Antigen ELISA) for the detection of M. tuberculosis in positive signals of Mycobacterium Growth Indicator Tubes (MGIT; BACTEC MGIT 960 system). DESIGN: A total of 208 consecutive clinical samples, including 185 respiratory specimens and 23 non-respiratory specimens, with positive signals in MGIT were analysed. The assay was performed on Days 1 and 7. The ELISA and conventional culture results were compared. RESULTS: Among the tubes with positive signals, 86 (41.3%) were M. tuberculosis, 55 (26.4%) were non-tuberculous mycobacteria and 67 (32.2%) were negative for mycobacteria. The sensitivity of the ELISA for tubes with positive signals (initial smear with positive acid-fast bacilli) on Days 1 and 7 was respectively 75.6% (70.5%) and 96.5% (97.7%), and the specificity was respectively 98.4% (100%) and 100% (100%). CONCLUSION: Our results show that the MeDiPro M. tuberculosis Antigen ELISA is a simple, rapid assay (<3 h) for M. tuberculosis antigen detection, especially on Day 7 of incubation with positive signals in the BACTEC MGIT 960 system. ? 2009 The Union. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/528672 | ISSN: | 1027-3719 | SDG/Keyword: | bacterial antigen; protein antibody; acid fast bacterium; article; bacterium detection; bacterium identification; comparative study; controlled study; culture technique; diagnostic accuracy; diagnostic value; enzyme linked immunosorbent assay; health center; human; human tissue; major clinical study; Mycobacterium tuberculosis; priority journal; respiratory system; sample; smear; Taiwan; tube; Antigens, Bacterial; Bacterial Proteins; Enzyme-Linked Immunosorbent Assay; Humans; Mycobacterium tuberculosis; Peptide Fragments; Sensitivity and Specificity |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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