https://scholars.lib.ntu.edu.tw/handle/123456789/529664
標題: | Hepatitis B virus surface antigen can activate dendritic cells and modulate T helper type immune response | 作者: | Jan R.-H. Lin Y.-L. Chen L.-K. Huang M.-T. LI-CHIEH WANG BOR-LUEN CHIANG |
公開日期: | 2011 | 卷: | 55 | 期: | 1 | 起(迄)頁: | 51-59 | 來源出版物: | Microbiology and Immunology | 摘要: | Hepatitis B virus surface antigen (HBsAg) is a major antigen of hepatitis B virus (HBV). Dendritic cells (DC) of HBV carriers have been reported to exhibit functional impairment. In this study, the role of HBsAg on mice bone marrow-derived dendritic cells and immune responses in vivo was studied. The immune modulatory function of HBsAg was explored by using mice bone marrow-derived dendritic cells in vitro and also by examining an ovalbumin (OVA) specific immune response in vivo. Treatment of dendritic cells with HBsAg resulted in enhanced cell surface expression of cluster of differentiation (CD) 80, CD83, CD86, and major histocompatibility complex (MHC) class II, and enhanced production of interleukin (IL)-12 p40 and IL-12 p70. Treatment of dendritic cells with HBsAg resulted in decreased T cell secretion of IL-5 by OVA stimulation. In addition, the results showed stronger OVA-specific immunoglobulin (Ig) M and weaker IgG responses in mice sera when they had been immunized with OVA and co-injected with HBsAg. It was also found that the mice exhibited significant enhancement of anti-OVA IgG2a antibody (Ab), as well as marked inhibition of IgG1 Ab production. In cellular immune responses, IL-5 production was significantly decreased and interferon (IFN)-γ increased in the group co-injected with HBsAg. On the other hand, the induction of lymphoproliferative response to OVA stimulation in spleen cells was decreased in the HBsAg co-injected group. These results demonstrate that HBsAg can affect the differentiation of T helper (Th) cells, which might provide a strategy for improving its prophylactic and therapeutic efficacy. ? 2010 The Societies and Blackwell Publishing Asia Pty Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650375250&doi=10.1111%2fj.1348-0421.2010.00284.x&partnerID=40&md5=da82e2f569e244e74e7d0d4d2ea7142a https://scholars.lib.ntu.edu.tw/handle/123456789/529664 |
ISSN: | 0385-5600 | DOI: | 10.1111/j.1348-0421.2010.00284.x | SDG/關鍵字: | B7 antigen; CD83 antigen; CD86 antigen; gamma interferon; hepatitis B surface antigen; immunoglobulin G1; immunoglobulin G2a; interleukin 10; interleukin 12p40; interleukin 12p70; interleukin 5; major histocompatibility antigen class 2; ovalbumin; animal cell; animal experiment; animal model; animal tissue; antibody production; antigen expression; article; cell activation; cell differentiation; cellular immunity; controlled study; cytokine production; dendritic cell; female; helper cell; hepatitis B; Hepatitis B virus; immune response; immunomodulation; in vitro study; in vivo study; lymphocyte proliferation; mouse; nonhuman; spleen cell; Th1 cell; Th2 cell; Animals; Cytokines; Dendritic Cells; Female; Hepatitis B Surface Antigens; Histocompatibility Antigens Class II; Immunoglobulin G; Interleukin-12; Interleukin-12 Subunit p40; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Ovalbumin; T-Lymphocytes, Helper-Inducer; Hepatitis B virus; Mus |
顯示於: | 醫學系 |
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