Heme oxygenase-1 gene promoter polymorphism and the risk of pediatric nonalcoholic fatty liver disease
Journal
International Journal of Obesity
Journal Volume
39
Journal Issue
8
Pages
1236-1240
Date Issued
2015
Author(s)
Abstract
Background and objectives:Oxidative stress and the insulin-resistant state are thought to be key components in the pathogenesis of pediatric nonalcoholic fatty liver disease (NAFLD). Heme oxygenase (HO) is important in the defense against oxidative stress. This study aimed to assess the association of HO-1 gene promoter polymorphism and insulin resistance with NAFLD among obese children.Methods:A total of 101 obese children aged 6-17 years were recruited. Anthropometric, serum biochemical variables and biomarkers for glucose and insulin metabolism were measured. We screened the allelic frequencies of (GT) n repeats in the HO-1 gene promoter among these obese children. NAFLD was determined through liver ultrasonography. Because the distribution of numbers of (GT) n repeats was bimodal, we divided the alleles into two classes: class S included shorter (27) repeats, and class L included longer (?27) repeats. We assessed the effects of the length of (GT) n repeats in HO-1 gene promoter on pediatric NAFLD.Results:Of the 101 obese subjects, 27 (26.7%) had NAFLD. The alanine aminotransferase level was higher in patients carrying L alleles (L/L and L/S) than patients with S alleles (S/S) (46.2±49.3 IU -1 versus 30.2±20.1 IU -1; P=0.027). The significant risk factors for pediatric NAFLD were patients carrying L alleles (L/L and L/S) (odds ratio (OR)=18.84; 95% confidence interval (CI): 1.45-245.22; P=0.025), homeostasis model assessment of insulin resistance (OR=1.40; 95% CI: 1.07-1.83; P=0.014) and age (OR=1.24; 95% CI: 1.03-1.50; P=0.025).Conclusion:In this hospital-based study, the obese children with longer GT repeats in the HO-1 gene promoter and insulin resistance were susceptible to NAFLD. ? 2015 Macmillan Publishers Limited. All rights reserved.
SDGs
Other Subjects
alanine aminotransferase; aspartate aminotransferase; bilirubin; biological marker; C reactive protein; gamma glutamyltransferase; genomic DNA; glucose; heme oxygenase 1; high density lipoprotein cholesterol; triacylglycerol; heme oxygenase 1; HMOX1 protein, human; microsatellite DNA; adolescent; age; alanine aminotransferase blood level; anthropometry; Article; aspartate aminotransferase blood level; bilirubin blood level; biochemical analysis; body mass; child; childhood disease; childhood obesity; cholesterol blood level; dinucleotide repeat; echography; female; gamma glutamyl transferase blood level; gene frequency; genetic association; genetic risk; glucose blood level; glucose metabolism; human; insulin metabolism; insulin resistance; liver examination; major clinical study; male; molecular pathology; nonalcoholic fatty liver; pediatric nonalcoholic fatty liver disease; priority journal; protein polymorphism; triacylglycerol blood level; waist circumference; complication; genetic polymorphism; genetic predisposition; genetics; Non-alcoholic Fatty Liver Disease; oxidative stress; Pediatric Obesity; promoter region; risk factor; Taiwan; Adolescent; Child; Female; Genetic Predisposition to Disease; Heme Oxygenase-1; Humans; Insulin Resistance; Male; Microsatellite Repeats; Non-alcoholic Fatty Liver Disease; Oxidative Stress; Pediatric Obesity; Polymorphism, Genetic; Promoter Regions, Genetic; Risk Factors; Taiwan
Publisher
Nature Publishing Group
Type
journal article