https://scholars.lib.ntu.edu.tw/handle/123456789/534265
標題: | Synergistic effect of polyethylenimine and cationic liposomes in nucleic acid delivery to human cancer cells | 作者: | Lee C.-H. YEN-HSUAN NI Chen C.-C. Chou C.-K. FU-HSIUNG CHANG |
公開日期: | 2003 | 出版社: | Elsevier | 卷: | 1611 | 期: | 1月2日 | 起(迄)頁: | 55-62 | 來源出版物: | Biochimica et Biophysica Acta - Biomembranes | 摘要: | Polyethylenimine (PEI) and other polycations are good vehicles for transferring genes into the cells. In earlier reports, poly-L-lysine and protamine have been shown to improve gene delivery with cationic liposomes. In this study, PEI, combined with different cationic liposomes, was studied to determine the optimal conditions for gene delivery. The reporter genes, luciferase and green fluorescent protein, were used to transfect human HeLa, HepG2 and hepatoma 2.2.15 cells with various combinations of PEIs (0.8 and 25 kDa), poly-L-lysine (15-30 kDa), protamine and cationic liposomes. The highest expression level was achieved by using the combination of PEI 25 kDa (0.65 μg/μg of DNA, nitrogen-to-DNA phosphate (N/P) ratio=4.5) with 10 nmol of DOTAP-cholesterol (DOTAP-Chol, 1:1 w/w). This DNA complex formulation dramatically increased the luciferase expression 10- to 100-fold, which was much higher than those of other polycations alone, cationic liposomes alone or the combination. In addition, PEI/DOTAP-Chol combination had little cytotoxicity than DOTAP-Chol or other cationic liposomes alone. The effect of oligonucleotide (ODN) delivery facilitated by PEI and cationic liposomes was also studied in the hepatoma cell lines. We demonstrated an antisense ODN of p53 delivered by PEI/DOTAP-Chol combination effectively inhibited the biosynthesis of p53 protein in HepG2 (68% inhibiton) and 2.2.15 cells (43% inhibition). Thus, the large PEI could synergistically increase the transfection efficiency when combined with the cationic liposomes. ? 2003 Elsevier Science B.V. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037376258&doi=10.1016%2fS0005-2736%2803%2900027-0&partnerID=40&md5=2866632aae0380204f265fc62f68a847 https://scholars.lib.ntu.edu.tw/handle/123456789/534265 |
ISSN: | 0005-2736 | DOI: | 10.1016/S0005-2736(03)00027-0 | SDG/關鍵字: | antisense oligodeoxynucleotide; cholesterol; DNA; green fluorescent protein; liposome; luciferase; oligodeoxynucleotide; phosphate; polyethyleneimine; protein p53; article; cell membrane transport; cell strain HepG2; controlled study; DNA drug complex; DNA transfer; drug cytotoxicity; gene expression; gene targeting; genetic transfection; HeLa cell; human; human cell; molecular weight; nonviral gene delivery system; priority journal; protein synthesis inhibition; reporter gene |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。