Bioactive compounds from the bark of Broussonetia papyrifera after solid fermentation with a white rot fungus Perenniporia tephropora
Journal
Journal of Wood Chemistry and Technology
Journal Volume
40
Journal Issue
5
Pages
317 - 330
Date Issued
2020
Author(s)
Abstract
In this study, potential bioactive compounds in the products of the solid fermentation of the paper mulberry tree bark (Broussonetia papyrifera) with a white rot fungus (Perenniporia tephropora) were investigated. Fractionation of methanolic extracts from the fermented products of the bark led to the isolation of 10 known compounds, namely broussonin B (1), broussonin A (2), 7,4'-dihydroxy-3'-prenylflavan (3), cathayanon H (4), broussoflavonol B (5), isoliquiritigenin (6), broussochalcone B (7), glyasperin A (8), marmesin (9), and vomifoliol (10). Their structures were determined by spectroscopic analysis (including considerations of MS, 1H, 13C NMR, COSY, HMQC, and HMBC spectra) and compared with structures that had been published in the literature. Of these compounds identified, cathayanon H (4) and glyasperin A (8) were isolated for the first time from the paper mulberry tree. Vomifoliol (10) was produced from the bark only after fermentation with white rot fungi. It was observed that concentrations of marmesin (9) and vomifoliol (10) generally increased with an increase in fermentation time, whereas concentration of compounds 1‒8 in the fermented products decreased during the same period. Biological tests showed that the tyrosinase inhibitory activity of marmesin (9) (IC50 = 168.0 μM) was similar to that of arbutin, a well-known tyrosinase inhibitor, whereas vomifoliol (10) exhibited no tyrosinase inhibition activity at any concentration higher than 2 mM. Accordingly, both solids and the liquid broth left after fermentation of the paper mulberry tree bark with the aforementioned white rot fungus could be developed for potential use in industrial applications.
SDGs
Type
journal article