https://scholars.lib.ntu.edu.tw/handle/123456789/535714
標題: | Dietary flavonoids luteolin and quercetin inhibit migration and invasion of squamous carcinoma through reduction of src/stat3/s100a7 signaling | 作者: | Fan, J.-J. Hsu, W.-H. Lee, K.-H. Chen, K.-C. Lin, C.-W. Lee, Y.-L.A. Ko, T.-P. Lee, L.-T. Lee, M.-T. Chang, M.-S. Cheng, C.-H. MAU-SUN CHANG |
關鍵字: | Luteolin; Metastasis; Quercetin; S100A7; Src; Stat3 | 公開日期: | 2019 | 卷: | 8 | 期: | 11 | 來源出版物: | Antioxidants | 摘要: | Flavonoids are well-known antioxidants and have shown the ability to prevent tumor formation and recurrence. Especially in dietary flavonoids, they have provided convenience and consistence of intake for long-term prevention of tumor formation. Previous reports suggested that S100 calcium-binding protein A7 (S100A7) might activate epithelial–mesenchymal transition (EMT) signaling and promote the metastasis of tumor cells; however, the regulatory signaling was unclear. In this study, we found that S100A7 was highly expressed in cancer cells and could be reduced by luteolin (Lu) and quercetin (Qu) through Src/Stat3 signaling. We found that the protein levels of S100A7, phosphorylated Src (p-Src), and p-Stat3 were increased in A431-III cells. Flavonoids Lu and Qu reduce protein levels of p-Src, p-Stat3 and S100A7 in A431-III cells. Treatment of A431-III cells with Src inhibitor SU6656 and Stat3 inhibitor S3I-201 also reduced the protein levels of S100A7. Transactivation activity of 5′-upstream regions of S100A7 was activated by Stat3 but was reduced by treatment with Lu, Qu, SU6656 and S3I-201. The treatment also reduced the migratory and invasive abilities of A431-III cells. In a further analysis of EMT markers, the protein level of E-cad increased and that of Twist decreased after treatment with the inhibitors and flavonoids. Overexpression of S100A7 decreased the protein level of E-cad and increased the Twist level, whereas knockdown of S100A7 had the opposite effects. Treatment with S3I-201, Lu and Qu, compared to the control, were found to decrease metastasis of tumor cells in zebrafish larvae. These results suggest that Lu and Qu may inhibit Src/Stat3/S100A7 signaling to reduce tumorigenesis of cancer cells. ? 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85075365650&partnerID=40&md5=84bf62d65e2f6b988db2cb4ff14862a5 https://scholars.lib.ntu.edu.tw/handle/123456789/535714 |
DOI: | 10.3390/antiox8110557 |
顯示於: | 生化科學研究所 |
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