https://scholars.lib.ntu.edu.tw/handle/123456789/535985
標題: | Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis | 作者: | Wu K.-M. Li N.-H. Yan J.-J. Tsao N. Liao T.-L. Tsai H.-C. Fung C.-P. Chen H.-J. Liu Y.-M. Wang J.-T. CHI-TAI FANG SHAN-CHWEN CHANG Shu H.-Y. Liu T.-T. Chen Y.-T. Shiau Y.-R. Lauderdale T.-L. Su I.-J. Kirby R. Tsai S.-F. |
公開日期: | 2009 | 卷: | 191 | 期: | 14 | 起(迄)頁: | 4492-4501 | 來源出版物: | Journal of Bacteriology | 摘要: | Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes liver abscesses was first observed in Asia and then was found worldwide. We are interested in how differences in gene content of the same species result in different diseases. Thus, we sequenced the whole genome of K. pneumoniae NTUH-K2044, which was isolated from a patient with liver abscess and meningitis, and analyzed differences compared to strain MGH 78578, which was isolated from a patient with pneumonia. Six major types of differences were found in gene clusters that included an integrative and conjugative element, clusters involved in citrate fermentation, lipopolysaccharide synthesis, and capsular polysaccharide synthesis, phage-related insertions, and a cluster containing fimbria-related genes. We also conducted comparative genomic hybridization with 15 K. pneumoniae isolates obtained from community-acquired or nosocomial infections using tiling probes for the NTUH-K2044 genome. Hierarchical clustering revealed three major groups of genomic insertion-deletion patterns that correlate with the strains' clinical features, antimicrobial susceptibilities, and virulence phenotypes with mice. Here we report the whole-genome sequence of K. pneumoniae NTUH-K2044 and describe evidence showing significant genomic diversity and sequence acquisition among K. pneumoniae pathogenic strains. Our findings support the hypothesis that these factors are responsible for the changes that have occurred in the disease profile over time. Copyright ? 2009, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-67650324665&doi=10.1128%2fJB.00315-09&partnerID=40&md5=c5d0ecbdde65383f10c09a80f7a0020e https://scholars.lib.ntu.edu.tw/handle/123456789/535985 |
ISSN: | 0021-9193 | DOI: | 10.1128/JB.00315-09 | SDG/關鍵字: | amoxicillin plus clavulanic acid; ampicillin; antibiotic agent; bacterial DNA; beta lactam antibiotic; cefalotin; cefazolin; cefotaxime; cefoxitin; ceftazidime; ceftriaxone; cefuroxime; cephalosporin; cephamycin; chloramphenicol; ciprofloxacin; cotrimoxazole; extended spectrum beta lactamase; genomic DNA; gentamicin; imipenem; levofloxacin; nalidixic acid; quinoline derived antiinfective agent; ribosome RNA; tetracycline; timentin; bacterial DNA; animal experiment; antibiotic sensitivity; article; Asia; bacterial gene; bacterial genome; bacterial meningitis; bacterial pneumonia; bacterial virulence; bacteriophage; bacterium isolation; carbohydrate synthesis; clinical feature; colony forming unit; comparative genomic hybridization; comparative study; controlled study; female; fimbria; gene cluster; hospital infection; human; Klebsiella pneumoniae; Klebsiella Pneumoniae NTUH K2044; liver abscess; minimum inhibitory concentration; mouse; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; strain difference; chemistry; cluster analysis; communicable disease; cross infection; DNA sequence; genetic variability; genetics; isolation and purification; Klebsiella infection; liver abscess; meningitis; microbiology; molecular genetics; nucleotide sequence; Klebsiella pneumoniae; Mus; Base Sequence; Cluster Analysis; Community-Acquired Infections; Comparative Genomic Hybridization; Cross Infection; DNA, Bacterial; Genetic Variation; Genome, Bacterial; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess; Meningitis; Molecular Sequence Data; Sequence Analysis, DNA |
顯示於: | 醫學系 |
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