Human interleukin-10 genotypes are associated with different precore/core gene mutation patterns in children with chronic hepatitis B virus infection
Journal
Journal of Pediatrics
Journal Volume
158
Journal Issue
5
Pages
808-813
Date Issued
2011
Author(s)
Lin Y.-T.
Lee T.-J.
Tsuei D.-J.
Abstract
Objective: To elucidate the association between human interleukin-10 (IL-10) genotypes and hepatitis B virus (HBV) precore/core gene mutation in children with chronic HBV infection. Study design: The study group comprised of 21 children with chronic HBV infection with spontaneous hepatitis B e antigen (HBeAg) seroconversion who were followed for more than 10 years. Another nine children without HBeAg seroconversion served as the control subjects. Sera at the immune tolerance and inflammatory phase (alanine aminotransferase, >80 IU/L) were subjected to HBV precore/core sequence analysis. IL-10 -1082 polymorphism was also determined. Results: HBV precore/core gene mutation increased significantly more in the inflammatory phase than in the tolerance phase (G1896A, 76.2% versus 4.8%; C1913A, 33.3% versus 0%; C2189A, 28.6% versus 4.8%; G2304A, 52.4% versus 14.3%) in study group (n = 21) but not the control group (n = 9). Subjects with the G/G genotype at the IL-10-1082 polymorphism site had higher C2189A mutation rate than the A allele carriers (P = .02). C2189A mutation carriers are associated with more viral load decrement from tolerance to inflammatory phase (P = .01) and earlier spontaneous HBeAg seroconversion (P = .01). Conclusions: The G/G genotype at the IL-10 -1082 polymorphism is associated with higher C2189A mutations, lower HBV viral load at immune inflammatory phase, and earlier spontaneous HBeAg seroconversion than A allele carriers. ? 2011 Mosby Inc. All rights reserved.
SDGs
Other Subjects
alanine aminotransferase; hepatitis B core antigen; hepatitis B(e) antigen; interleukin 10; adult; article; clinical article; controlled study; female; gene mutation; genotype; hepatitis B; Hepatitis B virus; human; immunological tolerance; inflammation; male; nonhuman; priority journal; seroconversion; single nucleotide polymorphism; virus load; Child, Preschool; Disease Progression; DNA; Female; Follow-Up Studies; Genotype; Hepatitis B e Antigens; Hepatitis B virus; Hepatitis B, Chronic; Humans; Interleukin-10; Male; Mutation; Polymerase Chain Reaction; Promoter Regions, Genetic; Time Factors; Viral Load
Type
journal article