https://scholars.lib.ntu.edu.tw/handle/123456789/537634
標題: | Morphometric analysis of erythrocytes from patients with thalassemia using tomographic diffractive microscopy | 作者: | Lin Y.-H. Huang S.-S. SHANG-JU WU Sung K.-B. |
關鍵字: | erythrocyte; thalassemia; three-dimensional adaptive region-growing; tomographic diffractive microscopy | 公開日期: | 2017 | 出版社: | SPIE | 卷: | 22 | 期: | 11 | 來源出版物: | Journal of Biomedical Optics | 摘要: | Complete blood count is the most common test to detect anemia, but it is unable to obtain the abnormal shape of erythrocytes, which highly correlates with the hematologic function. Tomographic diffractive microscopy (TDM) is an emerging technique capable of quantifying three-dimensional (3-D) refractive index (RI) distributions of erythrocytes without labeling. TDM was used to characterize optical and morphological properties of 172 erythrocytes from healthy volunteers and 419 erythrocytes from thalassemic patients. To efficiently extract and analyze the properties of erythrocytes, we developed an adaptive region-growing method for automatically delineating erythrocytes from 3-D RI maps. The thalassemic erythrocytes not only contained lower hemoglobin content but also showed doughnut shape and significantly lower volume, surface area, effective radius, and average thickness. A multi-indices prediction model achieved perfect accuracy of diagnosing thalassemia using four features, including the optical volume, surface-area-to-volume ratio, sphericity index, and surface area. The results demonstrate the ability of TDM to provide quantitative, hematologic measurements and to assess morphological features of erythrocytes to distinguish healthy and thalassemic erythrocytes. ? 2017 Society of Photo-Optical Instrumentation Engineers (SPIE). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038444355&doi=10.1117%2f1.JBO.22.11.116009&partnerID=40&md5=fbee578cab3496fb51274a4b6131e648 https://scholars.lib.ntu.edu.tw/handle/123456789/537634 |
ISSN: | 1083-3668 | DOI: | 10.1117/1.JBO.22.11.116009 | SDG/關鍵字: | Adaptive optics; Diagnosis; Refractive index; Tomography; Adaptive region growing; Complete blood counts; erythrocyte; Morphological features; Morphological properties; thalassemia; Threedimensional (3-d); Tomographic; Blood; cell shape; cytology; diagnostic imaging; erythrocyte; human; microscopy; thalassemia; tomography; Cell Shape; Erythrocytes; Humans; Microscopy; Thalassemia; Tomography |
顯示於: | 醫學系 |
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