https://scholars.lib.ntu.edu.tw/handle/123456789/539721
標題: | Correlation between serum lipid profiles and the ratio and count of the CD16+ monocyte subset in peripheral blood of apparently healthy adults | 作者: | ZEI-SHUNG HUANG Ching B.-L. |
關鍵字: | Atherosclerosis; CD16; HDL-cholesterol; LDL-cholesterol; Monocytes | 公開日期: | 2002 | 卷: | 101 | 期: | 1 | 起(迄)頁: | 11-17 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background and Purpose: Deposited vascular oxidized low-density lipoproteins (LDLs) are important triggers of the transformation of circulatory monocytes into macrophages. CD16+ monocytes have been reported to be the precursors of tissue macrophages. In this study, we sought to determine the relationship between serum LDL-cholesterol and the percentage and count of the CD16+ monocyte subset in the peripheral blood of healthy adults. Methods: We studied the correlations between serum lipid profiles and both peripheral CD16+ and CD36+ monocyte subset ratios and counts in apparently healthy adults (50 men and 50 women). Monocyte surface antigens CD16 and CD36 on CD14+ monocytes were detected using fluorescent triple staining and flow-cytometry. Surface staining was performed by incubating 1 × 106 blood mononuclear cells with phycocrythrin-conjugated anti-CD14, fluorescein isothiocyanate-conjugated anti-CD36, and respective control isotopes (mouse IgGs). A total of 5,000 cells were counted and the frequency of surface antigens was determined by FACscan. Results: A significant positive link between LDL-cholesterol and the CD16+ subset ratio was found by linear correlation analysis (p < 0.05) but not by multivariate regression analysis. Both linear correlation analysis and ANOVA revealed a significant inverse link between high-density lipoprotein (HDL)-cholesterol and the CD16+ subset ratio (both p < 0.01). By multivariate regression analysis, gender was the main significant determinant for the CD16+ subset ratio. When serum total cholesterol (TC) was excluded from the analysis to avoid the interference from collinearity between serum TC and LDL (r = 0.84), HDL-cholesterol became independently and inversely linked to the CD16+ subset ratio. There were independent inverse links between HDL-cholesterol and the counts of all monocytes, CD16+ monocytes, and CD36+ monocytes. Conclusions: Our results suggest that circulating HDL-cholesterol may be much more important than LDL-cholesterol in affecting the transformation of circulatory monocytes into macrophages. The inverse link between HDL-cholesterol and the number of macrophage precursors in peripheral blood might contribute partly to the well-known antiatherogenic effect of HDL-cholesterol. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036120142&partnerID=40&md5=d8a0efb07b83b2bfc383c39007aabce1 https://scholars.lib.ntu.edu.tw/handle/123456789/539721 |
ISSN: | 0929-6646 | SDG/關鍵字: | CD14 antigen; CD16 antigen; CD36 antigen; cholesterol; fluorescein isothiocyanate; fluorescent dye; high density lipoprotein cholesterol; immunoglobulin class; immunoglobulin G; low density lipoprotein cholesterol; membrane antigen; adult; analysis of variance; antigen detection; article; atherogenesis; blood; cell subpopulation; cell transformation; cholesterol blood level; controlled study; correlation analysis; female; flow cytometry; fluorescent antibody technique; gender; human; human cell; human experiment; leukocyte count; macrophage; male; monocyte; multivariate logistic regression analysis; normal human; stem cell; Antigens, CD36; Female; Humans; Leukocyte Count; Lipids; Male; Middle Aged; Monocytes; Receptors, IgG; Reference Values |
顯示於: | 醫學系 |
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