|Title:||Mutational signature of aristolochic acid exposure as revealed by whole-exome sequencing||Authors:||Hoang M.L.
|Issue Date:||2013||Journal Volume:||5||Journal Issue:||197||Start page/Pages:||197ra102||Source:||Science Translational Medicine||Abstract:||
In humans, exposure to aristolochic acid (AA) is associated with urothelial carcinoma of the upper urinary tract (UTUC). Exome sequencing of UTUCs from 19 individuals with documented exposure to AA revealed a remarkably large number of somatic mutations and an unusual mutational signature attributable to AA. Most of the mutations (72%) in these tumors were A:T-to-T:A transversions, located predominantly on the nontranscribed strand, with a strong preference for deoxyadenosine in a consensus sequence (T/CAG). This trinucleotide motif overlaps the canonical splice acceptor site, possibly accounting for the excess of splice site mutations observed in these tumors. The AA mutational fingerprint was found frequently in oncogenes and tumor suppressor genes in AA-associated UTUC. The AA mutational signature was observed in one patient's tumor from a UTUC cohort without previous indication of AA exposure. Together, these results directly link an established environmental mutagen to cancer through genome-wide sequencing and highlight its power to reveal individual exposure to carcinogens. Copyright 2013 by the American Association for the Advancement of Science.
|ISSN:||1946-6234||DOI:||10.1126/scitranslmed.3006200||metadata.dc.subject.other:||aristolochic acid; deoxyadenosine; DNA; trinucleotide; adult; aged; article; clinical article; cohort analysis; consensus sequence; controlled study; drug exposure; exome; female; gene mutation; gene sequence; human; human tissue; male; mutational signature; nucleic acid base substitution; oncogene; priority journal; RNA splicing; single nucleotide polymorphism; smoking; somatic mutation; Taiwan; TP53 gene; transitional cell carcinoma; tumor suppressor gene; Aged; Aristolochic Acids; Exome; Female; Humans; Male; Middle Aged; Mutation; Sequence Analysis, DNA; Urologic Neoplasms; Urothelium
|Appears in Collections:||醫學系|
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