https://scholars.lib.ntu.edu.tw/handle/123456789/542179
標題: | DNA binding activity of nuclear factor of activated T cells in mononuclear cells from renal transplant patients with and without BK virus viruria | 作者: | Yin W.-Y. Lai N.-S. Lee M.-C. CHIA-LI YU Huang S.-L. Lu M.-C. |
關鍵字: | BK virus; Calcineurin nuclear factor of activated T cells; Renal transplantation; Tacrolimus | 公開日期: | 2013 | 卷: | 25 | 期: | 2 | 起(迄)頁: | 112-116 | 來源出版物: | Tzu Chi Medical Journal | 摘要: | Objectives: Renal transplant patients receive calcineurin inhibitors to suppress the calcineurin-nuclear factor of activated T cells (NFAT) pathway. The DNA binding activity of NFAT and its relationship to the reactivation of BK virus (BKV) has not been evaluated in renal transplant patients. Patients and Methods: The DNA binding activity of NFAT cytoplasmic 1 (NFATc1) was measured by enzyme-linked immunosorbent assay in peripheral blood mononuclear cells from 26 renal transplant patients and 26 healthy controls. At the same time, their urinary BKV viral load was measured by real-time polymerase chain reaction. Results: The activity of NFATc1 was lower in renal transplant patients without BKV viruria [BKV (-)] than in healthy controls, while it trended to be higher in renal transplant patients with BKV viruria [BKV (+)] than in BKV (-) patients. The tacrolimus blood levels did not differ between BKV (+) and BKV (-) renal transplant patients or correlate with NFATc1 activity. Conclusion: NFATc1 DNA binding activity was lower in renal transplant patients without BKV viruria than in those who were BKV (+). However, there was no relationship between tacrolimus blood levels and NFATc1 activity in renal transplant patients. ? 2013, Buddhist Compassion Relief Tzu Chi Foundation. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878152925&doi=10.1016%2fj.tcmj.2013.04.001&partnerID=40&md5=11c289cfeddb4d9ff6d8163dfd33b4f1 https://scholars.lib.ntu.edu.tw/handle/123456789/542179 |
ISSN: | 1016-3190 | DOI: | 10.1016/j.tcmj.2013.04.001 | SDG/關鍵字: | cyclosporin; mycophenolic acid; mycophenolic acid 2 morpholinoethyl ester; prednisolone; rapamycin; tacrolimus; transcription factor NFAT; transcription factor NFATc1; unclassified drug; adult; age distribution; article; BK virus; bk virus infection; clinical article; controlled study; correlation analysis; DNA binding; enzyme linked immunosorbent assay; female; gene dosage; graft recipient; human; kidney transplantation; male; peripheral blood mononuclear cell; real time polymerase chain reaction; sex difference; virus load; virus reactivation |
顯示於: | 醫學系 |
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