|Title:||Prognosis of non-small cell lung cancer patients by detecting circulating cancer cells in the peripheral blood with multiple marker genes||Authors:||Sher Y.-P.
|Issue Date:||2005||Journal Volume:||11||Journal Issue:||1||Start page/Pages:||173-179||Source:||Clinical Cancer Research||Abstract:||
Purpose: Current lung cancer staging and prognosis methods are based on imaging methods, which may not be sensitive enough for early and accurate detection of metastasis. This study aims to validate the use of a panel of markers for circulating cancer cell detection to improve the accuracy of cancer staging, prognosis, and as a rapid assessment of therapeutic response. Experimental Design: We analyzed the National Cancer Institute-Cancer Genome Anatomy Project database to identify potential marker genes for the detection of circulating cancer cells in peripheral blood. Nested real-time quantitative PCR and a scoring method using cancer cell load Lc were employed to correlate the amount of circulating cancer cells with clinical outcomes in 54 non-small cell lung cancer (NSCLC) patients. The Kaplan-Meier method was employed for analysis of prognostic variables. Results: A panel of four marker genes was identified and experimentally validated. With these marker genes, we achieved an overall positive detection rate of 72% for circulating cancer cells in the peripheral blood of NSCLC patients. Patients who had higher Lc values had worse outcomes and shorter survival times. Patients with poor therapeutic response were revealed by positive detection of circulating cancer cells after therapy. The results correlated well with the patients' survival time. Conclusion: Circulating cancer cell detection by a panel of markers and the Lc scoring method can supplement the current tumor, node, metastasis staging method for improved prognosis and for rapid assessment of therapeutic response. Together, they may facilitate the design of better therapeutic strategies for the treatment of NSCLC patients.
|ISSN:||1078-0432||SDG/Keyword:||antineoplastic agent; accuracy; adult; aged; article; cancer cell; cancer staging; cancer survival; controlled study; correlation analysis; data base; female; human; human cell; human experiment; Kaplan Meier method; lung non small cell cancer; lymph node metastasis; male; marker gene; metastasis; peripheral circulation; priority journal; prognosis; quantitative analysis; real time polymerase chain reaction; scoring system; survival time; treatment outcome; validation process; Aged; Carcinoma, Non-Small-Cell Lung; Databases; DNA Primers; Female; Humans; Lung Neoplasms; Male; Middle Aged; Neoplasm Circulating Cells; Neoplasm Metastasis; Neoplasms; Polymerase Chain Reaction; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; RNA; Time Factors; Treatment Outcome; Tumor Markers, Biological
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.