https://scholars.lib.ntu.edu.tw/handle/123456789/542232
標題: | Arsenic Induces Human Keratinocyte Apoptosis by the FAS/FAS Ligand Pathway, Which Correlates with Alterations in Nuclear Factor-κB and Activator Protein-1 Activity | 作者: | Liao W.-T. Chang K.-L. CHIA-LI YU Chen G.-S. Chang L.W. Yu H.-S. |
關鍵字: | Cytotoxicity; Death receptor; Sodium arsenite; Transcription factor | 公開日期: | 2004 | 出版社: | Blackwell Publishing Inc. | 卷: | 122 | 期: | 1 | 起(迄)頁: | 125-129 | 來源出版物: | Journal of Investigative Dermatology | 摘要: | Epidemiologic studies demonstrated that long-term exposure to arsenic induces arsenical skin cancers, including Bowen's disease. Immunohistochemically, Bowen's disease shows proliferating and apoptotic characteristics. The transcription factors nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) functionally regulate cell proliferation, transformation, and apoptosis. To investigate the mechanism of arsenic-induced apoptosis and related alterations in NF-κB and AP-1 activity, we exposed cultured human foreskin keratinocytes to different concentrations of sodium arsenite. At lower concentrations (?1 μM), arsenic induced keratinocyte proliferation and enhanced both NF-κB and AP-1 activity. At higher concentrations (?5 μM), arsenic induced keratinocyte apoptosis by the Fas/Fas ligand (FasL) pathway. At apoptosis induction concentrations, NF-κB activity was not enhanced; however, AP-1 activity was further enhanced. These results indicated that upregulation of NF-κB at lower arsenic concentrations was correlated with keratinocyte proliferation. In contrast, higher concentrations of arsenic enhanced AP-1 and induced Fas/FasL-associated apoptosis. The concentration-dependent arsenic effects on transcription factors activity can help to clarify the mechanisms in arsenic-induced proliferation and apoptosis in keratinocytes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-1642534509&doi=10.1046%2fj.0022-202X.2003.22109.x&partnerID=40&md5=b720e62952b87d16358aa815f8462d16 https://scholars.lib.ntu.edu.tw/handle/123456789/542232 |
ISSN: | 0022-202X | DOI: | 10.1046/j.0022-202X.2003.22109.x | SDG/關鍵字: | arsenic; arsenite sodium; Fas antigen; Fas ligand; immunoglobulin enhancer binding protein; transcription factor AP 1; apoptosis; article; cell culture; cell proliferation; concentration (parameters); controlled study; correlation analysis; exposure; human; human cell; keratinocyte; prepuce; priority journal; protein analysis; upregulation |
顯示於: | 醫學系 |
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