https://scholars.lib.ntu.edu.tw/handle/123456789/543309
標題: | Reversible detection of phosphorylation and dephosphorylation by tip-enhanced Raman spectroscopy using a cyclopentadienyl ruthenium nanotag functionalized tip | 作者: | Lin, Y.-C. Ke, Z.-Y. Liao, P.-H. Tseng, C.-Y. Kong, K.V. KIEN-VOON KONG |
公開日期: | 2020 | 卷: | 56 | 期: | 6 | 起(迄)頁: | 936-939 | 來源出版物: | Chemical Communications | 摘要: | The detection of cancer invasion is crucial for diagnosis. In this report, we employed a TERS tip and SERS nanotags to create a cell signaling based nano-sensing system. This system is capable of creating a reversible phosphorylation/de-phosphorylation cycle for TERS measurement. The reversible TERS sensing is then paired with a downstream binding domain, Src homology region 2 (SH2), which is associated with the cell signaling for cancer cell invasion. Such a system offers the advantages of convenient detection of nanotags and high sensitivity as validated in a cell model. ? 2020 The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85078373531&partnerID=40&md5=792cd1e1a494926cfa1ad7be41c7363a https://scholars.lib.ntu.edu.tw/handle/123456789/543309 |
DOI: | 10.1039/c9cc08269g | SDG/關鍵字: | cyclopentadienyl ruthenium; nanoparticle; nanotag; phosphatase; protein SH2; ruthenium derivative; unclassified drug; Article; cancer cell; cell invasion; chemical structure; controlled study; human; human cell; MCF-7 cell line; MDA-MB-231 cell line; nonhuman; phosphorylation and dephosphorylation; protein binding; protein domain; sensitivity analysis; signal transduction; surface enhanced Raman spectroscopy; transmission electron microscopy; Yersinia pseudotuberculosis |
顯示於: | 化學系 |
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