https://scholars.lib.ntu.edu.tw/handle/123456789/543825
標題: | Enhanced uropathogenic Escherichia coli-induced infection in uroepithelial cells by sugar through TLR-4 and JAK/STAT1 signaling pathways | 作者: | Ho C.-H. Fan C.-K. Wu C.-C. HONG-JENG YU Liu H.-T. Chen K.-C. SHIH-PING LIU Cheng P.-C. |
公開日期: | 2019 | 出版社: | Elsevier Ltd | 來源出版物: | Journal of Microbiology, Immunology and Infection | 摘要: | Background: Patients with diabetes mellitus (DM) have higher incidence and more severe urinary tract infections (UTIs) for longer duration than those of the patients without DM. It causes more complicated etiologies during uropathogenic Escherichia coli (UPEC) infection. However, studies regarding the molecular mechanism are scarce. Methods: The present study (1) aimed to verify if sugar influences the process of UPEC-induced cystitis and invasion into the uroepithelial cells and (2) illustrated the mechanism of effects for sugar enhanced the UPEC infection into uroepithelial cells is related to TLR-4-mediated and JAK/STAT1-dependent pathway. Results: The results of the present study indicated that sugar can enhance UPEC infection in uroepithelial cells by up-regulating the transduced circuit between TLR-4-mediated UPEC interaction and JAK/STAT-1 signal pathways. The results of the inhibitor-co-incubating experiments demonstrated that the mechanism involved in the synergistic amplification of TLR-4-mediated UPEC interaction and JAK/STAT1 signaling pathways is responsible for the increased UPEC infection in uroepithelial cells. Conclusion: The results also proved that STAT-1 plays a critical role in the regulation of UPEC invasion and infection in the uroepithelial cells, especially those pretreated with glucose. The present study suggests a possible therapeutic approach to preferentially suppress UPEC infection during UTIs in the patients with diabetes. ? 2019 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068435538&doi=10.1016%2fj.jmii.2019.05.008&partnerID=40&md5=7bb94f2188d8dda0e5f3429a7fdd2b7f https://scholars.lib.ntu.edu.tw/handle/123456789/543825 |
ISSN: | 1684-1182 | DOI: | 10.1016/j.jmii.2019.05.008 | SDG/關鍵字: | carbohydrate; STAT1 protein; STAT1 protein, human; toll like receptor 4; cell line; epithelium cell; Escherichia coli infection; gene expression regulation; genetics; human; metabolism; microbiology; pathology; signal transduction; urinary tract infection; uropathogenic Escherichia coli; urothelium; Cell Line; Epithelial Cells; Escherichia coli Infections; Gene Expression Regulation; Humans; Signal Transduction; STAT1 Transcription Factor; Sugars; Toll-Like Receptor 4; Urinary Tract Infections; Uropathogenic Escherichia coli; Urothelium |
顯示於: | 醫學系 |
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