αB-Crystallin in clear cell renal cell carcinoma: Tumor progression and prognostic significance
Journal
Urologic Oncology: Seminars and Original Investigations
Journal Volume
31
Journal Issue
7
Pages
1367-1377
Date Issued
2013
Author(s)
Ho P.-Y.
Chiou S.-H.
Lee I.-L.
Yang H.-Y.
Peng H.-C.
Lai M.-K.
Abstract
Objectives: AlphaB-crystallin (αB-crystallin), a small heat shock protein, has been reported to be involved in the growth, antiapoptosis, migration, and chemoresistance of human malignancies. Materials and methods: αB-crystallin expression in normal renal and clear cell renal cell carcinoma (ccRCC) tissues was examined with two-dimensional (2D) gel electrophoresis assays. Immunohistochemistry was conducted to determine the presence of αB-crystallin-positive tumor cells and staining intensity in 50 cases of ccRCC tissue samples. The association of αB-crystallin protein expression, clinicopathogic parameters and prognosis of ccRCC patients was also analyzed with Student's t-test and Kaplan-Meier analysis. Moreover, Western blot assays were performed to detect the protein expression of αB-crystallin in normal and tumor tissues and the alteration of cell cycle regulators in αB-crystallin-overexpressing cells. MTT (3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide), BrdU, and transwell assays were performed to demonstrate the effects of αB-crystallin overexpression on cell growth, DNA synthesis and cell migration of ccRCC cells, respectively. Results: The results showed the up-regulation of αB-crystallin expression in ccRCC tissues. Overall survival of ccRCC patients was significantly correlated with αB-crystallin expression in tumor tissues. We found that αB-crystallin overexpression increased the expression of cyclin A and the incorporation of BrdU, which may be related to the enhancement of cell growth. Transwell analyses demonstrated that presence of αB-crystallin overexpression enhanced cell migration in ccRCC cells. Furthermore, rapamycin-resistance of tumor cells was induced when αB-crystallin was overexpressed. Conclusions: Our experimental findings highlight the importance of αB-crystallin in the tumor growth, migration, and target therapy-resistance of ccRCC cells. ? 2013 Elsevier Inc.
SDGs
Other Subjects
alpha crystallin; alphab crystallin; unclassified drug; adult; aged; apoptosis; article; cancer growth; cancer prognosis; cell migration; clinical article; controlled study; DNA synthesis; drug resistance; female; human; human cell; human tissue; immunohistochemistry; kidney carcinoma; male; migration; overall survival; priority journal; protein expression; two dimensional gel electrophoresis; upregulation; Clear cell renal cell carcinoma; Drug resistance; Migration; Prognostic factor; Proliferation; αB-crystallin; Adult; Aged; Aged, 80 and over; alpha-Crystallin B Chain; Blotting, Western; Carcinoma, Renal Cell; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cell Survival; Cyclin A; Disease Progression; Drug Resistance, Neoplasm; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Kidney Neoplasms; Male; Middle Aged; Multivariate Analysis; Prognosis; Proteomics; Sirolimus
Type
journal article