https://scholars.lib.ntu.edu.tw/handle/123456789/544323
標題: | The polymorphism XRCC1 Arg194Trp and 8-hydroxydeoxyguanosine increased susceptibility to arsenic-related renal cell carcinoma | 作者: | Hsueh Y.-M. Lin Y.-C. Chen W.-J. CHAO-YUAN HUANG Shiue H.-S. YEONG-SHIAU PU Chen C.-H. Su C.-T. |
公開日期: | 2017 | 出版社: | Academic Press Inc. | 卷: | 332 | 起(迄)頁: | 1-7 | 來源出版物: | Toxicology and Applied Pharmacology | 摘要: | This study was designed to explore the relationship between X-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms and renal cell carcinoma (RCC) and to investigate whether individuals with an XRCC1 risk genotype, a high level of 8-OHdG or a high urinary total arsenic concentration have a modified odds ratio (OR) of RCC. We recruited 180 RCC patients and 360 age- and sex-matched controls from a hospital-based pool. Image-guided biopsy or surgical resection of renal tumors was performed on RCC patients for pathological verification. Genomic DNA was used to examine the genotype of XRCC1(Arg399Gln), XRCC1(Arg194Trp), XRCC3(Thr241Met) and XPD(Lys751Gln) by PCR-RFLP. Liquid chromatography with tandem mass spectrometry was used to determine urinary 8-OHdG levels. A HPLC-HG-AAS was used to determine the concentrations of urinary arsenic species. Participants with the genotype XRCC1(Arg194Trp) Arg/Trp?+?Trp/Trp had a significantly higher OR of RCC than those with the Arg/Arg genotype; the OR and 95% confidence interval was 0.66 (0.45–0.97) after multivariate adjustment. The OR of RCC for the combined effect of high urinary 8-OHdG levels and high urinary total arsenic concentration in individuals with a XRCC1(Arg194Trp) Arg/Trp?+?Trp/Trp genotype was higher than in patients with an Arg/Arg genotype, which was evident in a dose response manner. In conclusion, this is the first study to show that the XRCC1 Arg194 allele is a predicting factor for RCC. The more risk factors (high urinary 8-OHdG levels, high urinary total arsenic concentrations, and XRCC1 Arg194 allele) that were present, the higher the OR of RCC. ? 2017 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85025811634&doi=10.1016%2fj.taap.2017.07.012&partnerID=40&md5=c0507f4ea89de311d75ae7b89f7ad41f https://scholars.lib.ntu.edu.tw/handle/123456789/544323 |
ISSN: | 0041-008X | DOI: | 10.1016/j.taap.2017.07.012 | SDG/關鍵字: | 8 hydroxydeoxyguanosine; arsenic; creatinine; genomic DNA; xeroderma pigmentosum group D protein; XRCC1 protein; XRCC3 protein; 8-oxo-7-hydrodeoxyguanosine; arsenic; deoxyguanosine; DNA binding protein; X-ray repair cross complementing protein 1; adult; allele; amino acid substitution; Article; cancer recurrence; cancer risk; cancer susceptibility; controlled study; creatinine blood level; DNA methylation; DNA polymorphism; female; genetic linkage; genotype; high performance liquid chromatography; human; human tissue; image guided biopsy; kidney biopsy; kidney carcinoma; liquid chromatography-mass spectrometry; major clinical study; male; metastasis; middle aged; restriction fragment length polymorphism; urine level; analogs and derivatives; body mass; case control study; genetic predisposition; genetics; genotyping technique; kidney tumor; questionnaire; renal cell carcinoma; risk factor; single nucleotide polymorphism; specimen handling; tandem mass spectrometry; urine; Alleles; Arsenic; Body Mass Index; Carcinoma, Renal Cell; Case-Control Studies; Deoxyguanosine; DNA-Binding Proteins; Female; Genetic Predisposition to Disease; Genotyping Techniques; Humans; Kidney Neoplasms; Male; Middle Aged; Polymorphism, Single Nucleotide; Risk Factors; Specimen Handling; Surveys and Questionnaires; Tandem Mass Spectrometry |
顯示於: | 醫學系 |
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