https://scholars.lib.ntu.edu.tw/handle/123456789/544432
標題: | Xanthine oxidase inhibitory triterpenoid and phloroglucinol from guttiferaceous plants inhibit growth and induced apoptosis in human ntub1 cells through a ROS-dependent mechanism | 作者: | Lin K.-W. Huang A.-M. Tu H.-Y. Lee L.-Y. Wu C.-C. Hour T.-C. Yang S.-C. YEONG-SHIAU PU Lin C.-N. |
關鍵字: | cytotoxicity; Garcinia subelliptica; Hypericum geminiflorum; reactive oxygen species; xanthine oxidase | 公開日期: | 2011 | 卷: | 59 | 期: | 1 | 起(迄)頁: | 407-414 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | A known triterpenoid, β-amyrin (1), and a known and a new phloroglucinol, cohulupone (2) and garcinielliptone P (3), were isolated from the pericarp and heartwood and seed of Garcinia subelliptica, respectively. A new xanthonolignoid, hyperielliptone HF (4), was isolated from the heartwood of Hypericum geminiflorum. The new compounds were established by analysis of their spectroscopic data. Compounds 1-3 showed an inhibitory effect on xanthine oxidase (XO). Treatment of NTUB1, a human bladder cancer cell, with 1 or 1 cotreated with cisplatin for 24 h resulted in a decreased viability of cells. Exposure of NTUB1 to 1 or 1 cotreated with cisplatin for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis indicated that treatment of NTUB1 with 1 or 1 cotreated with cisplatin led to the cell cycle arrest, accompanied by an increase in the extent of apoptotic cell death in 1 or 1 combined with cisplatin-treated NTUB1 after 24 h. These data suggested that the presentation of cell cycle arrest and apoptosis in 1 or 1 combined with cisplatin-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 1 or 1 cotreated with cisplatin. ? 2010 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78651061221&doi=10.1021%2fjf1041382&partnerID=40&md5=fca77c3664bac5befc1d284ebef78e29 https://scholars.lib.ntu.edu.tw/handle/123456789/544432 |
ISSN: | 0021-8561 | DOI: | 10.1021/jf1041382 | SDG/關鍵字: | Apoptosis; Apoptotic cell death; Cell-cycle arrest; Cis-platin; Flow-cytometric analysis; Garcinia subelliptica; Human bladder cancer; Hypericum geminiflorum; In-cell; Induced apoptosis; Inhibitory effect; Phloroglucinol; Reactive oxygen species; Spectroscopic data; Triterpenoids; Xanthine oxidase; Cell death; Data flow analysis; Oxygen; Spectroscopic analysis; Wood products; Platinum compounds; amyrin; cisplatin; drug derivative; enzyme inhibitor; oleanolic acid; phloroglucinol; plant extract; reactive oxygen metabolite; xanthine oxidase; apoptosis; article; bladder tumor; cell cycle; cell proliferation; chemistry; down regulation; drug antagonism; drug effect; Garcinia; human; metabolism; pathophysiology; tumor cell line; Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cisplatin; Down-Regulation; Enzyme Inhibitors; Garcinia; Humans; Oleanolic Acid; Phloroglucinol; Plant Extracts; Reactive Oxygen Species; Urinary Bladder Neoplasms; Xanthine Oxidase; Garcinia subelliptica; Hypericum geminiflorum |
顯示於: | 醫學系 |
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