Phloroglucinols Inhibit Chemical Mediators and Xanthine Oxidase, and Protect Cisplatin-Induced Cell Death by Reducing Reactive Oxygen Species in Normal Human Urothelial and Bladder Cancer Cells
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
57
Journal Issue
19
Pages
8782-8787
Date Issued
2009
Author(s)
Lin K.-W.
Huang A.-M.
Tu H.-Y.
Weng J.-R.U.
Hour T.-C.
Wei B.-L.
Yang S.-C.
Wang J.-P.
Lin C.-N.
Abstract
Phloroglucinols, garcinielliptones HA-HE (1-5), and C (6) were studied in vitro for their inhibitory effects on chemical mediators released from mast cells, neutrophils, and macrophages. Compound 6 revealed significant inhibitory effect on release of lysozyme from rat neutrophils stimulated with formyl-Met-Leu-Phe (fMLP)/cytochalasin B (CB). Compounds 3, 4, and 6 showed significant inhibitory effects on superoxide anion generation in rat neutrophils stimulated with (fMLP)/(CB), while compounds 1 and 5 revealed inhibitory effects on tumor necrosis factor-α (TNF-α) formation in macrophages stimulated with lipopolysaccharide (LPS). Compounds 1 and 3-6 showed inhibitory effects on xanthine oxidase (XO) and could inhibit the DNA breakage caused by O2-.. Treatment of NTUB1 with 2 to 60 μM compound 3 and 5 μM cisplatin and SV-HUC1 with 9 to 60 μM 3 and 5 μM cisplatin, respectively, resulted in an increase of viability of cells. These results indicated that compounds 1 and 3-6 showed anti-inflammatory effects and antioxidant activities. Compound 3 mediates through the suppression of XO activity and reduction of reactive oxygen species (ROS), and protection of subsequent cell death. ? 2009 American Chemical Society.
Subjects
Antioxidant; Garcinielliptones ha-he and anti-inflammatory; NTUB1; Phloroglucinol; SV-HUC1
SDGs
Other Subjects
antiinflammatory agent; antioxidant; cisplatin; enzyme inhibitor; garcinielliptone; phloroglucinol; reactive oxygen metabolite; triterpene; xanthine oxidase; animal; article; bladder tumor; cell death; chemistry; drug antagonism; drug effect; enzymology; Garcinia; human; immunology; macrophage; mast cell; neutrophil; pathology; rat; tumor cell line; urothelium; Animals; Anti-Inflammatory Agents; Antioxidants; Cell Death; Cell Line, Tumor; Cisplatin; Enzyme Inhibitors; Garcinia; Humans; Macrophages; Mast Cells; Neutrophils; Phloroglucinol; Rats; Reactive Oxygen Species; Triterpenes; Urinary Bladder Neoplasms; Urothelium; Xanthine Oxidase; Rattus
Type
journal article