https://scholars.lib.ntu.edu.tw/handle/123456789/544513
Title: | Cytotoxicity of arsenic trioxide to transitional carcinoma cells | Authors: | YEONG-SHIAU PU Hour T.-C. Chen J. CHAO-YUAN HUANG Guan J.-Y. Lu S.-H. |
Issue Date: | 2002 | Journal Volume: | 60 | Journal Issue: | 2 | Start page/Pages: | 346-350 | Source: | Urology | Abstract: | Objectives. To explore the therapeutic efficacy of arsenic trioxide (As2O3) in human transitional cell carcinomas, we investigated the potential use of the compound as a chemotherapeutic agent and the possible cross-resistance with cisplatin in this malignancy. Methods. Three bladder transitional carcinoma cell lines, NTUB1, NTUB1/P (cisplatin-resistant), and NTUB1/As (As2O3-resistant), were used. The chemosensitivity of the three cell lines to cisplatin and As2O3 was determined by the microculture tetrazolium assay. The modulatory effect of buthionine sulfoximine (BSO) on As2O3 cytotoxicity was studied by combining the two agents simultaneously or sequentially and evaluated using the median-effect analysis. Cellular glutathione contents were determined using a biochemical method. Results. There was evident cross-resistance between cisplatin and As2O3 in the cell model used. BSO significantly enhanced As2O3 cytotoxicity in the three cell lines, indicating synergism in combination. In the presence of 3 μM BSO, the sensitivity of NTUB1, NTUB1/P, and NTUB1/As to As2O3 was increased 3, 7.4, and 8.4-fold, respectively. Among the three different combination schedules, greater cytotoxic effects were obtained by concurrent exposure to both agents. A significant dose-response relationship was found between the BSO concentrations and glutathione contents in NTUB1 (P = 0.007) and NTUB1/As (P = 0.05) but not NTUB1/P (P = 0.1) cells. Conclusions. As2O3 in the presence of BSO may be an active agent against transitional cell carcinoma. Our results have clinical implications and warrant further investigation. ? 2002, Elsevier Science Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036321065&doi=10.1016%2fS0090-4295%2802%2901699-0&partnerID=40&md5=b716b43995f91e1d0827b010c486631b https://scholars.lib.ntu.edu.tw/handle/123456789/544513 |
ISSN: | 0090-4295 | DOI: | 10.1016/S0090-4295(02)01699-0 | SDG/Keyword: | arsenic trioxide; buthionine sulfoximine; cisplatin; glutathione; analytic method; article; assay; cancer cell culture; cancer chemotherapy; chemosensitivity; concentration response; controlled study; cross resistance; cytotoxicity; drug effect; drug efficacy; drug exposure; drug potentiation; drug screening; human; human cell; model; priority journal; protein content; transitional cell carcinoma; Antineoplastic Agents; Arsenicals; Buthionine Sulfoximine; Carcinoma, Transitional Cell; Cisplatin; Drug Resistance, Neoplasm; Drug Screening Assays, Antitumor; Glutathione; Humans; Oxides; Tumor Cells, Cultured; Urinary Bladder Neoplasms |
Appears in Collections: | 醫學系 |
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