https://scholars.lib.ntu.edu.tw/handle/123456789/545329
標題: | Hepatitis B virus core antigen determines viral persistence in a C57BL/6 mouse model | 作者: | Lin Y.-J. Huang L.-R HUNG-CHIH YANG Tzeng H.-T PING-NING HSU Wu H.-L PEI-JER CHEN DING-SHINN CHEN |
公開日期: | 2010 | 出版社: | National Academy of Sciences | 卷: | 107 | 期: | 20 | 起(迄)頁: | 9340-9345 | 來源出版物: | Proceedings of the National Academy of Sciences of the United States of America | 摘要: | We recently developed a mouse model of hepatitis B virus (HBV) persistence, in which a single i.v. hydrodynamic injection of HBV DNA to C57BL/6 mice allows HBV replication and induces a partial immune response, so that about 20-30% of the mice carry HBV for more than 6 months. The model was used to identify the viral antigen crucial for HBV persistence. We knocked out individual HBV genes by introducing a premature termination codon to the HBV core, HBeAg, HBx, and polymerase ORFs. The specific-gene-deficient HBV mutants were hydrodynamically injected into mice and the HBV profiles of the mice were monitored. About 90% of the mice that received the HBcAg-mutated HBV plasmid exhibited high levels of hepatitis B surface antigenemia and maintained HBsAg expression for more than 6 months after injection. To map the region of HBcAg essential for viral clearance, we constructed a set of serial HBcAg deletion mutants for hydrodynamic injection. We localized the essential region of HBcAg to the carboxyl terminus, specifically to the 10 terminal amino acids (HBcAg176-185). The majority of mice receiving this HBV mutant DNA did not elicit a proper HBcAg-specific IFN-γ response and expressed HBV virions for 6 months. These results indicate that the immune response triggered in mice by HBcAg during exposure to HBV is important in determining HBV persistence. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983727018&doi=10.1073%2fpnas.1004762107&partnerID=40&md5=03c629026f9e6d1c3da6ff604b6f1418 https://scholars.lib.ntu.edu.tw/handle/123456789/545329 |
ISSN: | 0027-8424 | DOI: | 10.1073/pnas.1004762107 | SDG/關鍵字: | gamma interferon; hepatitis B core antigen; hepatitis B surface antigen; hepatitis B virus X protein; hepatitis B(e) antigen; virus DNA; animal cell; animal experiment; animal model; animal tissue; antibody titer; antigen expression; article; C57BL 6 mouse; codon; controlled study; deletion mutant; gene construct; hepatitis B; Hepatitis B virus; humoral immunity; male; mouse; nonhuman; open reading frame; persistent virus infection; plasmid; priority journal; spleen cell; virion; virus core; virus mutant; virus replication; virus transcription; Hepatitis B virus; Mus |
顯示於: | 醫學系 |
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