|Title:||α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer||Authors:||Hsu P.-I.
|Issue Date:||2007||Journal Volume:||13||Journal Issue:||3||Start page/Pages:||876-883||Source:||Clinical Cancer Research||Abstract:||
Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001 and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that α1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies. ? 2007 American Association for Cancer Research.
|ISSN:||1078-0432||DOI:||10.1158/1078-0432.CCR-06-1404||SDG/Keyword:||alpha 1 antitrypsin; alpha 1 antitrypsin; peptide; protein precursor; trypsin; tumor marker; article; controlled study; duodenum ulcer; human; mass spectrometry; polyacrylamide gel electrophoresis; priority journal; protein analysis; proteomics; stomach cancer; stomach juice; stomach ulcer; two dimensional gel electrophoresis; adult; biosynthesis; chemistry; duodenum ulcer; female; male; mass spectrometry; metabolism; methodology; middle aged; pH; risk; stomach; stomach juice; stomach tumor; ulcer; Adult; alpha 1-Antitrypsin; Duodenal Ulcer; Electrophoresis, Gel, Two-Dimensional; Female; Gastric Juice; Humans; Hydrogen-Ion Concentration; Male; Mass Spectrometry; Middle Aged; Odds Ratio; Peptides; Protein Precursors; Proteomics; Stomach; Stomach Neoplasms; Trypsin; Tumor Markers, Biological; Ulcer
|Appears in Collections:||醫學系|
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