https://scholars.lib.ntu.edu.tw/handle/123456789/545735
標題: | Monitoring the effect of mechanical stress on mesenchymal stem cell collagen production by multiphoton microscopy | 作者: | Chen, W.-L. Chang, C.-C. Chiou, L.-L. Li, T.-H. Liu, Y. Lee, Hsuan-Shu CHEN-YUAN DONG |
公開日期: | 2008 | 卷: | 6858 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | Tissue engineering is emerging as a promising method for repairing damaged tissues. Due to cartilage's common wear and injury, in vitro production of cartilage replacements have been an active area of research. Finding the optimal condition for the generation of the collagen matrix is crucial in reproducing cartilages that closely match those found in human. Using multiphoton autofluorescence and second-harmonic generation (SHG) microscopy we monitored the effect of mechanical stress on mesenchymal stem cell collagen production. Bone marrow mesenchymal stem cells in the form of pellets were cultured and periodically placed under different mechanical stress by centrifugation over a period of four weeks. The differently stressed samples were imaged several times during the four week period, and the collagen production under different mechanical stress is characterized. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-45549097504&doi=10.1117%2f12.763227&partnerID=40&md5=62e3fdedac5a526dfa8cec76f07bc9c7 https://scholars.lib.ntu.edu.tw/handle/123456789/545735 |
ISSN: | 1605-7422 | DOI: | 10.1117/12.763227 | SDG/關鍵字: | Biotechnology; Cartilage; Cell culture; Cells; Centrifugation; Codes (symbols); Collagen; Harmonic generation; Health; Ligaments; Microscopes; Optics; Pelletizing; Photoacoustic effect; Stresses; Technology; Tissue culture; Tissue engineering; active areas; Autofluorescence (AF); Bone marrow mesenchymal stem cells (BMSC); Collagen matrices; In vitro production; mechanical stressing; Mesenchymal stem cells; Multi-photon; Multiphoton microscopy (MPM); Optimal conditions; Regenerative medicines; Second harmonic generation (SHG); Tissue |
顯示於: | 醫學系 |
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