https://scholars.lib.ntu.edu.tw/handle/123456789/545867
Title: | Packaging of hepatitis delta virus RNA via the RNA-binding domain of hepatitis delta antigens: Different roles for the small and large delta antigens | Authors: | Wang H.-W. PEI-JER CHEN Lee, Cha-Ze Wu H.L. DING-SHINN CHEN |
Issue Date: | 1994 | Publisher: | American Society for Microbiology | Journal Volume: | 68 | Journal Issue: | 10 | Start page/Pages: | 6363-6371 | Source: | Journal of Virology | Abstract: | Hepatitis delta virus (HDV) is composed of four specific components. The first component is envelope protein which contains hepatitis B surface antigens. The second and third components are nucleocapsid proteins, referred to as small and large hepatitis delta antigens (HDAgs). The final component is a single-stranded circular RNA molecule known as the viral genome. In order to study the mechanism of HDV RNA packaging, a four-plasmid cotransfection system in which each viral component was provided by a separate plasmid was employed. Virus-like particles released from Huh-7 cells receiving such a cotransfection were found to contain HDV RNA along with three proteins. Therefore, the four-plasmid cotransfection system could lead to successful HDV RNA packaging in vitro. The system was then used to show that the large HDAg alone was able to achieve a low level of HDV RNA packaging. Analysis of a variety of large HDAg mutants revealed that the RNA- binding domain was essential for viral RNA packaging. By increasing the incorporation of small HDAg into virus-like particles, we found a three- to fourfold enhancement of HDV RNA packaging. This effect was probably through a direct binding of HDV RNA, independent from that of large HDAg, with the small HDAg. The subsequent RNA-protein complex was packaged into particles. The results provided insight into the roles and functional domains of small and large HDAgs in HDV RNA packaging. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983719875&doi=10.1128%2fjvi.68.10.6363-6371.1994&partnerID=40&md5=b621a17d5ab297581f069887ba7bf7e6 https://scholars.lib.ntu.edu.tw/handle/123456789/545867 |
ISSN: | 0022-538X | DOI: | 10.1128/jvi.68.10.6363-6371.1994 | SDG/Keyword: | RNA binding protein; virus antigen; antigen expression; article; binding affinity; delta agent hepatitis; DNA binding; DNA transfection; gene expression; genome; Hepatitis delta virus; immunoblotting; Northern blotting; priority journal; virus nucleocapsid; virus particle; virus replication |
Appears in Collections: | 醫學系 |
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