https://scholars.lib.ntu.edu.tw/handle/123456789/545886
標題: | Emblica officinalis gaertn. attentuates N-nitrosodiethylamine-induced apoptosis, autophagy, and inflammation in rat livers | 作者: | Chen K.-H. BOR-RU LIN Chien C.-T. Ho C.-H. |
關鍵字: | antioxidant; apoptosis; autophagy; inflammation; liver injury; reactive oxygen species | 公開日期: | 2011 | 卷: | 14 | 期: | 44385 | 起(迄)頁: | 746-755 | 來源出版物: | Journal of Medicinal Food | 摘要: | Inflammation and oxidative stress contribute to liver injury. Amla (Emblica officinalis Gaertn.) is rich in vitamin C, gallic acid, flavonoids, and tannins, which may protect against hepatoxicity-induced liver injury. We elucidated the effects of supplementary Amla (100 mg/kg of body weight) on N-nitrosodiethylamine-induced injury by evaluating reactive oxygen species (ROS) responses in the liver and bile, the degree of accumulated leukocytes and Kupffer cell infiltration, 3-nitrotyrosine and 4-hydroxynonenal stains, apoptosis and autophagy, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transpeptidase (γ-GT) levels, and antioxidant/oxidant enzymes in rats. Amla was more potent than vitamin C in scavenging O 2 -?, hydrogen peroxide, and nitric oxide. N-Nitrosodiethylamine increased ROS production in liver and bile, hepatic Kupffer cell and leukocyte infiltration, 3-nitrotyrosine and 4-hydroxynonenal accumulations, apoptosis and autophagy, and plasma ALT, AST, and γ-GT levels in the rats, decreased hepatic manganese superoxide dismutase (MnSOD) and catalase protein expressions, and enhanced inducible nitric oxide synthase (iNOS) and cytochrome P450 2E1 (CYP2E1) protein expressions. Amla significantly preserved MnSOD and catalase expressions and decreased iNOS and CYP2E1 protein expressions in N-nitrosodiethylamine-treated livers. Amla decreased N-nitrosodiethylamine-enhanced hepatic apoptosis and autophagy appearances via down-regulation of the Bax/Bcl-2 ratio and Beclin-1 expression. Thus Amla supplementation counteracts N-nitrosodiethylamine-induced liver injury via its antioxidant, anti-inflammation, anti-apoptosis, and anti-autophagy properties. ? 2011, Mary Ann Liebert, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960458862&doi=10.1089%2fjmf.2010.1459&partnerID=40&md5=0603ebc872146963c5172c1a707244a5 https://scholars.lib.ntu.edu.tw/handle/123456789/545886 |
ISSN: | 1096-620X | DOI: | 10.1089/jmf.2010.1459 | SDG/關鍵字: | 3 nitrotyrosine; 4 hydroxynonenal; alanine aminotransferase; ascorbic acid; aspartate aminotransferase; beclin 1; catalase; cytochrome P450 2E1; diethylnitrosamine; Emblica officinalis extract; gamma glutamyltransferase; hydrogen peroxide; inducible nitric oxide synthase; manganese superoxide dismutase; nitric oxide; poly(adenosine diphosphate ribose); protein Bax; protein bcl 2; reactive oxygen metabolite; SunAmla; unclassified drug; alanine aminotransferase blood level; animal cell; animal experiment; animal model; animal tissue; apoptosis; article; aspartate aminotransferase blood level; autophagy; bile; cell infiltration; controlled study; down regulation; drug effect; Emblica officinalis; gamma glutamyl transferase blood level; hepatitis; Kupffer cell; leukocyte; liver; liver metabolism; male; nonhuman; priority journal; protein expression; rat; Animals; Antioxidants; Apoptosis; Autophagy; Diethylnitrosamine; Disease Models, Animal; Down-Regulation; gamma-Glutamyltransferase; Humans; Liver; Liver Neoplasms; Male; Nitric Oxide Synthase Type II; Oxidative Stress; Phyllanthus emblica; Plant Extracts; Rats; Rats, Wistar; Superoxide Dismutase; Phyllanthus emblica; Rattus |
顯示於: | 醫學系 |
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