Simultaneous analysis of multiclass antibiotic residues in complex environmental matrices by liquid chromatography with tandem quadrupole mass spectrometry
Journal
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Journal Volume
1145
Date Issued
2020
Author(s)
Abstract
A simultaneous extraction and cleanup method was optimized and validated for the determination of 40 antibiotics from cephalosporin, fluoroquinolone, lincosamide, macrolide, nitroimidazole, quinolone, sulfonamide and tetracycline groups in sediments by liquid chromatography with tandem quadrupole mass spectrometry (LC-MS/MS). The method involved hydration of freeze-dried sediment sample (2.0 g) with 2.5 ml of 0.1 M Na-EDTA McIlvaine buffer and extraction with 5 ml of MeOH and MeCN (1:3 v/v) followed by dispersive solid phase extraction by using 100 mg mix of C18 and PSA (1:2 w/w) and 50 mg MgSO4 prior to LC-MS/MS analysis. The method was validated for 10, 20, 50 and 100 µg/kg spiking levels by using blank sediment sample obtained from a drinking water reservoir according to the guidelines of European Commission Decision (2002) 2002/657/EC. The method produced clean extracts with generally low matrix effect during LC-MS/MS analysis. The mean recoveries ranged between 24–162%, 48–151%, 51–159%, and 50–149% for 10, 20, 50 and 100 µg/kg spiking levels, respectively, with acceptable precision. The analytical method was sensitive enough to achieve 0.01–34.3 µg/kg and 0.03–115 µg/kg limits of detection and quantitation, respectively. The scope of the method was demonstrated by analyzing complex solid environmental matrices (chicken manure, swine manure, poultry feed and soil) spiked at 10, 20, 50 and 100 µg/kg levels. The method was also applied for the antibiotic analysis in samples with incurred residues. Different matrices in the order of the magnitude as sediments < poultry feed < swine manure < soil < chicken manure were detected with the residues of fluoroquinolone, macrolide, sulfonamide and tetracycline antibiotics. © 2020 Elsevier B.V.
Subjects
Antibiotics; Cleanup; Complex solid matrices; Extraction; Liquid chromatography tandem mass spectrometry; Sediments
Other Subjects
Animals; Antibiotics; Fertilizers; Liquid chromatography; Magnesium compounds; Manures; Mass spectrometry; Phase separation; Potable water; Reservoirs (water); Sediments; Sewage treatment plants; Sulfur compounds; Dispersive solid phase extraction; Environmental matrixes; European Commission; Limits of detection and quantitation; Multi-class antibiotics; Simultaneous analysis; Simultaneous extractions; Tetracycline antibiotics; Extraction; antibiotic agent; cephalosporin derivative; lincosamide; macrolide; nitroimidazole; quinolone derivative; sulfonamide; tetracycline derivative; antiinfective agent; drug residue; fresh water; Article; limit of detection; limit of quantitation; liquid chromatography-mass spectrometry; matrix effect; poultry manure; priority journal; quadrupole mass spectrometry; solid phase extraction; swine manure; tandem mass spectrometry; water supply; animal; animal food; chemistry; chicken; high performance liquid chromatography; isolation and purification; manure; pig; procedures; sediment; soil pollutant; tandem mass spectrometry; water pollutant; Animal Feed; Animals; Anti-Bacterial Agents; Chickens; Chromatography, High Pressure Liquid; Drug Residues; Fresh Water; Geologic Sediments; Manure; Soil Pollutants; Solid Phase Extraction; Swine; Tandem Mass Spectrometry; Water Pollutants, Chemical
Type
journal article