A Multifunctional Microfluidic Device for Blood Typing and Primary Screening of Blood Diseases
Journal
ACS Sensors
Journal Volume
5
Journal Issue
10
Pages
3082-3090
Date Issued
2020
Author(s)
Abstract
In this work, we demonstrate a multifunctional, portable, and disposable microfluidic device for blood typing and primary screening of blood diseases. Preloaded antibodies (anti-A, anti-B, and anti-D) interact with injected whole blood cells to cause an agglutination reaction that blocks a microslit in the microfluidic channel to accumulate red blood cells and form a visible red line that can be easily read to determine the blood type. Moreover, the different blood density and agglutination properties of normal and subtype blood groups, as well as different blood diseases, including anemia and polycythemia vera, generate different lengths of blood agglutination within the channels, which allows us to successfully screen these various conditions in as little as 2 min. The required blood volume for each test is just 1 μL, which can be obtained by minimally invasive finger pricking. This novel method of observing agglutinated red blood cells to distinguish blood types and diseases is both feasible and affordable, suggesting its promise for use in areas with limited resources. ?
Subjects
anemia; blood agglutination test; blood typing; point-of-care diagnosis; polycythemia
SDGs
Other Subjects
Antigen-antibody reactions; Bacteria; Cells; Diagnosis; Fluidic devices; Microfluidics; Blood typing; Blood volumes; Micro-fluidic devices; Microfluidic channel; Minimally invasive; Polycythemia vera; Primary screening; Red blood cell; Blood; antigen antibody reaction; blood group typing; hematologic disease; human; lab on a chip; microfluidics; Agglutination; Blood Grouping and Crossmatching; Hematologic Diseases; Humans; Lab-On-A-Chip Devices; Microfluidics
Type
journal article