https://scholars.lib.ntu.edu.tw/handle/123456789/547200
標題: | Cord blood stem-cell-derived dendritic cells generate potent antigen-specific immune responses and anti-tumour effects | 作者: | Chang M.-C. CHIEN-NAN LEE YU-LI CHEN YING-CHENG CHIANG WEI-ZEN SUN Hu Y.-H. CHI-AN CHEN WEN-FANG CHENG |
公開日期: | 2012 | 卷: | 123 | 期: | 6 | 起(迄)頁: | 347-360 | 來源出版物: | Clinical Science | 摘要: | The aim of the present study was to investigate whether CBSCs [(umbilical) cord blood stem cells] can be a new source of DCs (dendritic cells), which can generate more potent antigen-specific immune responses and anti-tumour effects. CBSCs and PBMCs (peripheral blood mononuclear cells) were collected, cultured and differentiated into DCs. Surface markers, secreting cytokines, antigen-presentation activity, antigen-specific cell-mediated immunity and cytotoxic killing effects induced by these two DC origins were evaluated and compared. CBSCs were expanded ~17-fold by ex vivo culture. The expression of surface markers in CBSC-derived DCs were higher than those in PBMC-derived DCs treated with LPS (lipopolysaccharide). The CBSC-derived DCs mainly secreted IL (interleukin)-6, IL-10 and TNF (tumour necrosis factor)-α, whereas PBMC-derived DCs mainly secreted IL-5 and IFN (interferon)-γ. The CBSC-derived DCs had better antigen-presentation abilities when stimulated with LPS or TNF-α, induced higher numbers of IFN-γ -secreting antigen-specific CD8 + T-cells, as assessed using an ELISpot (enzymelinked immunosorbent spot) assay, and stimulated more potent antigen-specific CTL (cytotoxic T-cell) activities (P<0.01, one-way ANOVA). CBSC-derived DCs had quicker and greater ERK (extracellular-signal-regulated kinase) and Akt phosphorylation, and weaker p38 phosphorylation, than PBMC-derived DCs when stimulated with LPS. In conclusion, CBSC-derived DCs have the ability to induce stronger antigen-specific immunity and more potent anti-tumour effects and therefore could be a good source of DCs for use in DC-based cancer vaccines and immunotherapy. ? The Authors Journal compilation ? 2012 Biochemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862586626&doi=10.1042%2fCS20110272&partnerID=40&md5=35603b0ca1df8c7b1823f659dbeb55c0 https://scholars.lib.ntu.edu.tw/handle/123456789/547200 |
DOI: | 10.1042/CS20110272 | SDG/關鍵字: | gamma interferon; granulocyte macrophage colony stimulating factor; interleukin 10; interleukin 4; interleukin 5; interleukin 6; lipopolysaccharide; mitogen activated protein kinase; protein kinase B; synaptophysin; tumor necrosis factor alpha; antigen specificity; article; CD8+ T lymphocyte; cord blood stem cell; cytotoxic T lymphocyte; dendritic cell; enzyme linked immunospot assay; enzyme phosphorylation; human; human cell; immune response; peripheral blood mononuclear cell; priority journal; stem cell; umbilical cord blood; Antigen Presentation; Cell Differentiation; Cell Line, Tumor; Culture Media; Cytokines; Dendritic Cells; Extracellular Signal-Regulated MAP Kinases; Fetal Blood; HeLa Cells; Humans; Immunity, Cellular; Immunotherapy; K562 Cells; Neoplasms; p38 Mitogen-Activated Protein Kinases; Phosphorylation; Proto-Oncogene Proteins c-akt; Signal Transduction; Stem Cells |
顯示於: | 醫學系 |
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