Cord blood stem-cell-derived dendritic cells generate potent antigen-specific immune responses and anti-tumour effects
Journal
Clinical Science
Journal Volume
123
Journal Issue
6
Pages
347-360
Date Issued
2012
Author(s)
Chang M.-C.
Hu Y.-H.
Abstract
The aim of the present study was to investigate whether CBSCs [(umbilical) cord blood stem cells] can be a new source of DCs (dendritic cells), which can generate more potent antigen-specific immune responses and anti-tumour effects. CBSCs and PBMCs (peripheral blood mononuclear cells) were collected, cultured and differentiated into DCs. Surface markers, secreting cytokines, antigen-presentation activity, antigen-specific cell-mediated immunity and cytotoxic killing effects induced by these two DC origins were evaluated and compared. CBSCs were expanded ~17-fold by ex vivo culture. The expression of surface markers in CBSC-derived DCs were higher than those in PBMC-derived DCs treated with LPS (lipopolysaccharide). The CBSC-derived DCs mainly secreted IL (interleukin)-6, IL-10 and TNF (tumour necrosis factor)-α, whereas PBMC-derived DCs mainly secreted IL-5 and IFN (interferon)-γ. The CBSC-derived DCs had better antigen-presentation abilities when stimulated with LPS or TNF-α, induced higher numbers of IFN-γ -secreting antigen-specific CD8 + T-cells, as assessed using an ELISpot (enzymelinked immunosorbent spot) assay, and stimulated more potent antigen-specific CTL (cytotoxic T-cell) activities (P<0.01, one-way ANOVA). CBSC-derived DCs had quicker and greater ERK (extracellular-signal-regulated kinase) and Akt phosphorylation, and weaker p38 phosphorylation, than PBMC-derived DCs when stimulated with LPS. In conclusion, CBSC-derived DCs have the ability to induce stronger antigen-specific immunity and more potent anti-tumour effects and therefore could be a good source of DCs for use in DC-based cancer vaccines and immunotherapy. ? The Authors Journal compilation ? 2012 Biochemical Society.
SDGs
Other Subjects
gamma interferon; granulocyte macrophage colony stimulating factor; interleukin 10; interleukin 4; interleukin 5; interleukin 6; lipopolysaccharide; mitogen activated protein kinase; protein kinase B; synaptophysin; tumor necrosis factor alpha; antigen specificity; article; CD8+ T lymphocyte; cord blood stem cell; cytotoxic T lymphocyte; dendritic cell; enzyme linked immunospot assay; enzyme phosphorylation; human; human cell; immune response; peripheral blood mononuclear cell; priority journal; stem cell; umbilical cord blood; Antigen Presentation; Cell Differentiation; Cell Line, Tumor; Culture Media; Cytokines; Dendritic Cells; Extracellular Signal-Regulated MAP Kinases; Fetal Blood; HeLa Cells; Humans; Immunity, Cellular; Immunotherapy; K562 Cells; Neoplasms; p38 Mitogen-Activated Protein Kinases; Phosphorylation; Proto-Oncogene Proteins c-akt; Signal Transduction; Stem Cells
Type
journal article