A Benzenoid 4,7-Dimethoxy-5-Methyl-L, 3-Benzodioxole from Antrodia cinnamomea Attenuates Dendritic Cell-Mediated Th2 Allergic Responses
Journal
American Journal of Chinese Medicine
Journal Volume
47
Journal Issue
6
Pages
1271
Date Issued
2019-01-01
Author(s)
Abstract
© 2019 World Scientific Publishing Company. Dendritic cells (DCs) play a critical role in initiating immune responses; however, DCs also induce Th2-related allergic sensitivities. Thus, DCs become a target for therapeutic design in allergic diseases. In this study, we aim to investigate the anti-allergic effect of pure compounds from a medicinal mushroom Antrodia cinnamomea (Ac) on DC-induced allergic responses. We identified a benzenoid compound 4,7-dimethoxy-5-methyl-l,3-benzodioxole (DMB) which may modulate Th2 polarization in bone marrow-derived DCs (BMDCs) and in a murine food allergy model. DMB effectively reduced the Th2 adjuvant cholera toxin (CT)-induced BMDC maturation and cytokine production. In studying the mechanism, DMB blocked the molecular processes involved in Th2 induction, including cAMP activation, IL-33 production, and IRF4/Tim4 upregulation, in CT-activated BMDCs. Furthermore, DMB treatment attenuated the symptoms, clinical scores, and Th2 responses of CT-induced ovalbumin (OVA)-specific food allergy in mice at sensitization stage. These results indicated that DMB could suppress DC function for Th2 polarization and mitigate allergic responses. Thus, DMB may have potential to be a novel agent for preventing or treating food allergy.
Subjects
Antrodia cinnamomea | Cholera Toxin | Dendritic Cell | Food Allergy | Th2
Antrodia cinnamomea; Cholera Toxin; Dendritic Cell; Food Allergy; Th2
SDGs
Other Subjects
4,7 dimethoxy 5 methyl levo,3 benzodioxole; antiallergic agent; aromatic compound; cholera toxin; cyclic AMP; interferon regulatory factor 4; interleukin 33; ovalbumin; plant extract; unclassified drug; 1,3 benzodioxole derivative; antiallergic agent; cytokine; animal cell; animal experiment; animal model; antiallergic activity; Antrodia camphorata; Article; bone marrow derived dendritic cell; cell maturation; controlled study; cytokine production; drug isolation; drug mechanism; drug synthesis; food allergy; in vitro study; in vivo study; mouse; nonhuman; polarization; sensitization; Th2 cell; upregulation; animal; Antrodia; Bagg albino mouse; cell culture; chemistry; dendritic cell; disease model; food allergy; hypersensitivity; immunology; metabolism; phytotherapy; Th2 cell; Animals; Anti-Allergic Agents; Antrodia; Benzodioxoles; Cells, Cultured; Cytokines; Dendritic Cells; Disease Models, Animal; Food Hypersensitivity; Hypersensitivity; Mice, Inbred BALB C; Ovalbumin; Phytotherapy; Th2 Cells
Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
Type
journal article