https://scholars.lib.ntu.edu.tw/handle/123456789/548404
標題: | Gene expression profiling of gastric cancer by microarray combined with laser capture microdissection | 作者: | MING-SHIANG WU Lin Y.-S. YU-TING CHANG CHIA-TUNG SHUN MING-TSAN LIN Lin J.-T. |
公開日期: | 2005 | 出版社: | WJG Press | 卷: | 11 | 期: | 47 | 起(迄)頁: | 7405-7412 | 來源出版物: | World Journal of Gastroenterology | 摘要: | Aim: To examine the gene expression profile of gastric cancer (GC) by combination of laser capture microdissection (LCM) and microarray and to correlate the profiling with histological subtypes. Methods: Using LCM, pure cancer cells were procured from 45 cancerous tissues. After procurement of about 5 000 cells, total RNA was extracted and the quality of RNA was determined before further amplification and hybridization. One microgram of amplified RNA was converted to cDNA and hybridized to cDNA microarray. Results: Among 45 cases, only 21 were qualified for their RNAs. A total of 62 arrays were performed. These included 42 arrays for cancer (21 cases with dye-swab duplication) and 20 arrays for non-tumorous cells (10 cases with dye-swab duplication) with universal reference. Analyzed data showed 504 genes were differentially expressed and could distinguish cancerous and non-cancerous groups with more than 99% accuracy. Of the 504 genes, trefoil factors 1, 2, and 3 were in the list and their expression patterns were consistent with previous reports. Immunohistochemical staining of trefoil factor 1 was also consistent with the array data. Analyses of the tumor group with these 504 genes showed that there were 3 subgroups of GC that did not correspond to any current classification system, including Lauren's classification. Conclusion: By using LCM, linear amplification of RNA, and cDNA microarray, we have identified a panel of genes that have the power to discriminate between GC and non-cancer groups. The new molecular classification and the identified novel genes in gastric carcinogenesis deserve further investigations to elucidate their clinicopathological significance. ? 2005 The WJG Press and Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144467863&doi=10.3748%2fwjg.v11.i47.7405&partnerID=40&md5=c7fb1c7a06746dff9ab44122b2724a22 https://scholars.lib.ntu.edu.tw/handle/123456789/548404 |
ISSN: | 1007-9327 | DOI: | 10.3748/wjg.v11.i47.7405 | SDG/關鍵字: | complementary DNA; RNA; article; cancer cell; cancer classification; cancer tissue; clinical article; controlled study; data analysis; diagnostic accuracy; DNA microarray; gene expression profiling; gene identification; histopathology; human; human tissue; immunohistochemistry; laser capture microdissection; nucleotide sequence; RNA extraction; RNA hybridization; stomach cancer; stomach carcinogenesis; tissue microarray; unindexed sequence |
顯示於: | 醫學系 |
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