https://scholars.lib.ntu.edu.tw/handle/123456789/548569
標題: | Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/ planar cell polarity pathway | 作者: | Lee, Y.-H. Kawakami, K. HuangFu, W.-C. I-HSUAN LIU |
公開日期: | 2020 | 卷: | 15 | 期: | 4 | 來源出版物: | PLoS ONE | 摘要: | Pericellular and extracellular proteoglycans play an important role in modulating morphogen gradients and signal transductions. Chondroitin sulfate proteoglycan 4 (Cspg4) is a membrane spanning proteoglycan expressed in immature progenitor cells and cancer cells. Cspg4 participates in cellular events such as proliferation, migration and signal transduction, and these events are generally important for embryo development. In this study, we characterized Cspg4 for its roles in zebrafish embryonic development. Our results demonstrated that cspg4 was maternally expressed from 0 to 3 hours post fertilization (hpf) and expressed in the anterior and posterior embryo end after 9 hpf. Knocking-down cspg4 resulted in a shorter anterior-posterior axis than control embryo, which could be rescued by co-injecting wnt11 mRNA suggesting that Cspg4 regulates body axis organization through modulating the Wnt/planar cell polarity signaling pathway. In addition, overexpressing cspg4 caused cyclopia. The Cspg4 transmembrane domain mutant embryo phenocopied the global overexpression of cspg4 mRNA and led to cyclopia with a very low penetrance. Our results demonstrated that the quantitatively and spatially accurate distribution of Cspg4 is critical for body axis and midline development during gastrulation. ? 2020 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85082802290&partnerID=40&md5=2e081c61e58cf17d94f69a82597cad48 https://scholars.lib.ntu.edu.tw/handle/123456789/548569 |
DOI: | 10.1371/journal.pone.0230943 | SDG/關鍵字: | chondroitin sulfate proteoglycan 4; messenger RNA; proteochondroitin sulfate; unclassified drug; Wnt protein; Wnt11 protein; antigen; chondroitin sulfate proteoglycan 4; proteoglycan; adult; animal experiment; animal tissue; anterior posterior axis; Article; axial patterning; cell polarity; controlled study; cspg4 gene; cyclopia; embryo; embryo development; gene; gene knockdown; gene location; gene overexpression; human; human cell; modulation; nonhuman; quantitative analysis; Wnt signaling; zebra fish; animal; cell polarity; metabolism; nonmammalian embryo; physiology; Wnt signaling; Animals; Antigens; Cell Polarity; Embryo, Nonmammalian; Embryonic Development; Proteoglycans; RNA, Messenger; Wnt Signaling Pathway; Zebrafish |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。