https://scholars.lib.ntu.edu.tw/handle/123456789/549979
標題: | Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software | 作者: | HSIAO-LIN HWA MING-YIH WU Chung, Wan-Chia TSANG-MING KO Lin, Chih-Peng Yin, Hsiang-I Lee, Tsui-Ting JAMES CHUN-I LEE |
關鍵字: | DNA mixture; EuroForMix; ForenSeq™ system; Massively parallel sequencing; Short tandem repeat; Single nucleotide polymorphism | 公開日期: | 一月-2019 | 出版社: | SPRINGER | 卷: | 133 | 期: | 1 | 起(迄)頁: | 25 | 來源出版物: | International journal of legal medicine | 摘要: | Massively parallel sequencing (MPS) technologies enable the simultaneous analysis of short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). MPS also enables the detection of alleles of the minor contributors in imbalanced DNA mixtures. In this study, 59 STRs (amelogenin, 27 autosomal STRs, 7 X-STRs, and 24 Y-STRs) and 94 identity-informative SNPs of 119 unrelated Taiwanese (50 men, 69 women) were sequenced using a commercial MPS kit. Forty-eight nondegraded and 44 highly degraded two-person artificial DNA mixtures with various minor to major ratios (1:9, 1:19, 1:29, 1:39, 1:79, and 1:99) were analyzed to examine the performance of this system for detecting the alleles of the minor contributors in DNA mixtures. Likelihood ratios based on continuous model were calculated using the EuroForMix for DNA mixture interpretation. The STR and SNP genotypes of these 119 Taiwanese were obtained. Several sequence variants of STRs were observed. Using EuroForMix software based on the sequence data of autosomal STRs and autosomal SNPs, 97.9% (47/48) and 97.7% (42/43) of minor donors were accurately inferred among the successfully analyzed nondegraded and degraded DNA mixtures, respectively. In conclusion, combined with EuroForMix software, this commercial kit is effective for assignment of the minor contributors in nondegraded and degraded DNA mixtures. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/549979 | ISSN: | 0937-9827 | DOI: | 10.1007/s00414-018-1961-y |
顯示於: | 醫學系 |
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