https://scholars.lib.ntu.edu.tw/handle/123456789/550494
標題: | Decoy receptor 3 increases monocyte adhesion to endothelial cells via NF-κB-dependent up-regulation of intercellular adhesion molecule-1, VCAM-1, and IL-8 expression | 作者: | CHIA-RON YANG Hsieh S.-L. Ho F.-M. WAN-WAN LIN |
公開日期: | 2005 | 卷: | 174 | 期: | 3 | 起(迄)頁: | 1647-1656 | 來源出版物: | Journal of Immunology | 摘要: | Decoy receptor 3 (DcR3), a soluble receptor for FasL, LIGHT and TL1A, is highly expressed in cancer cells. We show that pretreatment of HUVECs with DcR3 enhances the adhesion of THP-1 and U937 cells and primary monocytes. A similar stimulatory effect of DcR3 on THP-1 adhesion was also observed in human microvascular endothelial cells (HMVECs). Flow cytometry and ELISA showed that DcR3-treated HUVECs exhibited significant increases in ICAM-1 and VCAM-1 expression. We also demonstrate the ability of DcR3 to stimulate the secretion of IL-8 by HUVECs. RT-PCR and reporter assays revealed that the expression of adhesion molecules and IL-8 are regulated at the level of gene transcription. Experiments with pyrrolidine dithiocarbamate indicated the involvement of an NF-κB signaling pathway. DcR3 was found to induce IκB kinase activation, IκB degradation, p65 nuclear translocation, and NF-κB DNA-binding activity. The enhancement by DcR3 of cell adhesion to HUVECs was not mimicked by the TL1A-Ab, which has been shown in our previous work to be a neutralizing Ab against TL1A, thereby inducing HUVECs angiogenesis. Moreover, DcR3-induced cell adhesion could be detected in human aortic endothelial cells (ECs) in which TL1A expression is lacking. Together, our data demonstrate that DcR3 increases monocyte adhesion to ECs via NF-κB activation, leading to the transcriptional up-regulation of adhesion molecules and IL-8 in ECs. This novel action appears not to be due to TL1A neutralization, but occurs through an as yet undefined target(s). This study implicates DcR3 in the relationship between inflammation and cancer development. |
URI: | 2-s2.0-12444330566 https://scholars.lib.ntu.edu.tw/handle/123456789/550494 |
ISSN: | 221767 | DOI: | 10.4049/jimmunol.174.3.1647 | SDG/關鍵字: | cell adhesion molecule; cell surface receptor; decoy receptor 3; I kappa B; immunoglobulin enhancer binding protein; interleukin 8; membrane protein; pyrrolidine dithiocarbamate; transcription factor; unclassified drug; vascular cell adhesion molecule 1; angiogenesis; aorta; article; assay; cancer cell; cell adhesion; DNA binding; endothelium cell; enzyme activation; enzyme linked immunosorbent assay; flow cytometry; genetic transcription; human; human cell; inflammation; microvasculature; monocyte; priority journal; protein degradation; secretion; signal transduction; transcription regulation; upregulation |
顯示於: | 藥理學科所 |
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