https://scholars.lib.ntu.edu.tw/handle/123456789/550770
標題: | Parenterally administered porcine epidemic diarrhea virus-like particle-based vaccine formulated with CCL25/28 chemokines induces systemic and mucosal immune protectivity in pigs | 作者: | Hsu, C.-W. Chang, M.-H. HUI-WEN CHANG Wu, T.-Y. YEN-CHEN CHANG |
關鍵字: | Chemokines; Pig; Porcine epidemic diarrhea virus; Vaccine; VLP | 公開日期: | 2020 | 卷: | 12 | 期: | 10 | 來源出版物: | Viruses | 摘要: | Generation of a safe, economical, and effective vaccine capable of inducing mucosal immunity is critical for the development of vaccines against enteric viral diseases. In the current study, virus-like particles (VLPs) containing the spike (S), membrane (M), and envelope (E) structural proteins of porcine epidemic diarrhea virus (PEDV) expressed by the novel polycistronic baculovirus expression vector were generated. The immunogenicity and protective efficacy of the PEDV VLPs formulated with or without mucosal adjuvants of CCL25 and CCL28 (CCL25/28) were evaluated in post-weaning pigs. While pigs intramuscularly immunized with VLPs alone were capable of eliciting systemic anti-PEDV S-specific IgG and cellular immunity, co-administration of PEDV VLPs with CCL25/28 could further modulate the immune responses by enhancing systemic anti-PEDV S-specific IgG, mucosal IgA, and cellular immunity. Upon challenge with PEDV, both VLP-immunized groups showed milder clinical signs with reduced fecal viral shedding as compared to the control group. Furthermore, pigs immunized with VLPs adjuvanted with CCL25/28 showed superior immune protection against PEDV. Our results suggest that VLPs formulated with CCL25/28 may serve as a potential PEDV vaccine candidate and the same strategy may serve as a platform for the development of other enteric viral vaccines. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85092329758&partnerID=40&md5=4d96072f84490f10da8dc8790514394c https://scholars.lib.ntu.edu.tw/handle/123456789/550770 |
DOI: | 10.3390/v12101122 | SDG/關鍵字: | bacmid; complementary DNA; gamma interferon; hexahistidine; immunoglobulin A; immunoglobulin G; neutralizing antibody; virus RNA; virus vaccine; vitronectin; beta chemokine; chemokine; immunological adjuvant; virus antibody; virus like particle vaccine; virus vaccine; animal experiment; animal model; antibody titer; Article; Baculoviridae; body weight; cell isolation; cellular immunity; controlled study; electron microscopy; enzyme linked immunospot assay; immune response; immunization; immunofluorescence; immunomodulation; indirect fluorescent antibody technique; nonhuman; peripheral blood mononuclear cell; pig; plasmid; porcine epidemic diarrhea; protein electrophoresis; protein expression; protein purification; real time polymerase chain reaction; RNA extraction; Sf21 cell line; transmission electron microscopy; ultracentrifugation; virus neutralization; virus shedding; Western blotting; animal; Coronavirus infection; feces; immunology; metabolism; mucosal immunity; Porcine epidemic diarrhea virus; Sf9 cell line; swine disease; vaccination; virology; Adjuvants, Immunologic; Animals; Antibodies, Viral; Chemokines; Chemokines, CC; Coronavirus Infections; Feces; Immunity, Cellular; Immunity, Mucosal; Porcine epidemic diarrhea virus; Sf9 Cells; Swine; Swine Diseases; Vaccination; Vaccines, Virus-Like Particle; Viral Vaccines; Virus Shedding |
顯示於: | 分子暨比較病理生物學研究所 |
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