Whole genomic analysis and comparison of two canine papillomavirus type 9 strains in malignant and benign skin lesions
Journal
Viruses
Journal Volume
12
Journal Issue
7
Date Issued
2020
Author(s)
Yamashita-Kawanishi, N.
Tomizawa, S.
Chen, W.-T.
Chang, Y.-C.
Shirota, K.
Chambers, J.K.
Uchida, K.
Haga, T.
Abstract
Papillomaviruses (PVs) usually cause benign proliferative lesions in the stratified epithelium of various animal species. However, some high-risk types of PVs have been proven to lead to malignant transformations. In dogs, several canine papillomaviruses (CPVs) have been identified in malignant lesions and are suggested as one of the risk factors for the development of squamous cell carcinomas (SCCs). In the present study, the full genomes of two CPV9 strains from recurrent SCCs of Dog 1 and skin viral papilloma (viral plaque) of Dog 2 were sequenced. Alignment of the two CPV9 sequences with the genome of the reference CPV9 strain (accession no. JF800656.1) derived from a solitary pigmented plaque was performed. Compared with the reference strain, a 27 bp in-frame insertion in the E1 gene was identified in both CPV9 strains in this study. In comparison with the CPV9 strains derived from benign lesions, the CPV9 from the SCCs of Dog 1 exhibited a 328 bp deletion at the 3' end of the E2 and spacer sequence, which encoded a truncated deduced E2 protein and a chimeric E8E2 protein. However, there was no difference in the mRNA expression levels of viral oncoproteins of E6 and E7 between the two CPV9 cases, suggesting that the oncogenesis of CPV9 for malignant transformation might be different from that of human papillomaviruses. The roles of E2 and E8E2 deleted CPV9 in the oncogenesis of benign and malignant lesions should be further investigated.
Other Subjects
glycoprotein E1; lactadherin; messenger RNA; oncoprotein; prostaglandin E2; RNA binding protein; RNA polymerase; virus DNA; animal experiment; animal model; Article; benign skin tumor; Canis; carcinogenesis; comparative study; controlled study; DNA extraction; DNA synthesis; gene expression; genetic analysis; histology; histopathology; immunohistochemistry; keratinization; mRNA expression level; nonhuman; papilloma; polymerase chain reaction; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; risk factor; RNA extraction; RNA isolation; sequence analysis; sequence homology; skin defect; Vaccinia virus; virus detection; virus strain; Wart virus; whole genome sequencing; animal; classification; dog; dog disease; genetics; genomics; male; Papillomaviridae; papillomavirus infection; pathogenicity; skin; skin tumor; squamous cell carcinoma; tumor recurrence; veterinary medicine; virology; virus genome; Animals; Carcinoma, Squamous Cell; DNA, Viral; Dog Diseases; Dogs; Genome, Viral; Genomics; Male; Neoplasm Recurrence, Local; Papillomaviridae; Papillomavirus Infections; Risk Factors; Skin; Skin Neoplasms; Whole Genome Sequencing
Type
journal article