https://scholars.lib.ntu.edu.tw/handle/123456789/550772
標題: | Intramuscular immunization with chemokine-adjuvanted inactive porcine epidemic diarrhea virus induces substantial protection in pigs | 作者: | Hsueh, F.-C. YEN-CHEN CHANG Kao, C.-F. Hsu, C.-W. HUI-WEN CHANG |
關鍵字: | Adjuvants; CC chemokine; Mucosal immunity; Porcine epidemic diarrhea virus | 公開日期: | 2020 | 卷: | 8 | 期: | 1 | 來源出版物: | Vaccines | 摘要: | Intramuscular (IM) immunization is generally considered incapable of generating a protective mucosal immune response. In the swine industry, attempts to develop a safe and protective vaccine for controlling porcine epidemic diarrhea (PED) via an IM route of administration have been unsuccessful. In the present study, porcine chemokine ligand proteins CCL25, 27, and 28 were constructed and stably expressed in the mammalian expression system. IM co-administration of inactivated PEDV (iPEDV) particles with different CC chemokines and Freund’s adjuvants resulted in recruiting CCR9+ and/or CCR10+ inflammatory cells to the injection site, thereby inducing superior systemic PEDV specific IgG, fecal IgA, and viral neutralizing antibodies in pigs. Moreover, pigs immunized with iPEDV in combination with CCL25 and CCL28 elicited substantial protection against a virulent PEDV challenge. We show that the porcine CC chemokines could be novel adjuvants for developing IM vaccines for modulating mucosal immune responses against mucosal transmissible pathogens in pigs. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85079851442&partnerID=40&md5=c6f05e57bfb1363d71a0e4eb68bc7c83 https://scholars.lib.ntu.edu.tw/handle/123456789/550772 |
DOI: | 10.3390/vaccines8010102 | SDG/關鍵字: | adjuvant; CCL25 chemokine; CCL28 chemokine; chemokine; chemokine receptor CCR10; chemokine receptor CCR9; cutaneous T cell attracting chemokine; immunoglobulin A; immunoglobulin G; inactivated vaccine; neutralizing antibody; unclassified drug; animal experiment; animal tissue; Article; controlled study; enzyme linked immunosorbent assay; feces analysis; genetic transfection; human; human cell; immune response; immunization; immunocytochemistry; immunohistochemistry; intramuscular drug administration; nonhuman; piglet; plasmid; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; protein expression; protein purification; virus inactivation; virus load; virus neutralization; Western blotting |
顯示於: | 分子暨比較病理生物學研究所 |
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