https://scholars.lib.ntu.edu.tw/handle/123456789/551264
標題: | Effect of 5-Aminolevulinic Acid-Mediated Photodynamic Therapy on MCF-7 and MCF-7/ADR Cells | 作者: | Tsai, Tsuimin RUEY-LONG HONG JUI-CHANG TSAI PEI-JEN LOU Ling, I-Fang Chen, Chin-Tin |
公開日期: | 2004 | 卷: | 34 | 期: | 1 | 起(迄)頁: | 62-72 | 來源出版物: | Lasers in Surgery and Medicine | 摘要: | Background and Objectives: Photodynamic therapy (PDT) has been proposed as an alternative approach in overcoming multidrug resistance (MDR) phenotype. To verify whether 5-aminolevulinic acid (ALA)-mediated PDT is effective in MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, and phototoxicity in human breast cancer cells MCF-7 and derived MDR subline, MCF-7/ADR. Study Design/Materials and Methods: The fluorescence kinetics of ALA-induced PpIX was evaluated by spectrofluorometer. The phototoxicity of MCF-7 and MCF-7/ADR cells was determined by tetrazolium (MTT) assays and clonogenic assay. Furthermore, Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after ALA-PDT. Results: MCF-7/ADR accumulated a lower level of PpIX as compared to parental MCF-7 cells. Significant phototoxicity was observed in MCF-7 and increased in a fluence-dependent manner with LD50 around 8 J/cm 2. Compared to its parental counterpart, MCF-7/ADR cells were less sensitive to ALA photodynamic treatment and PDT-induced cytotoxicity did not increase in a dose responsive manner as the concentration of ALA increased or the fluence of light increased. ALA-PDT was less effective for MCF-7/ADR cells than MCF-7 cells even under the condition when these two cell lines contained the similar amounts of PpIX. Conclusions: These results indicate that, except for the MDR related characteristics, MCF-7/ADR cells might possess intrinsic mechanisms that render them less sensitive to ALA-PDT induced phototoxicity. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347762872&doi=10.1002%2flsm.10246&partnerID=40&md5=e96ca3642a64983c6958f37bc569f1db https://scholars.lib.ntu.edu.tw/handle/123456789/551264 |
ISSN: | 0196-8092 | DOI: | 10.1002/lsm.10246 | SDG/關鍵字: | 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; aminolevulinic acid; lipocortin 5; propidium iodide; protoporphyrin; article; cell line; cell strain MCF 7; cellular distribution; clonogenic assay; controlled study; cytotoxicity; dose response; fluorescence; human; human cell; light; multidrug resistance; photodynamic therapy; photosensitivity; phototoxicity; priority journal; spectrofluorometry; Aminolevulinic Acid; Cell Death; Cell Line, Tumor; Humans; Mitochondria; Photochemotherapy; Photosensitizing Agents |
顯示於: | 腫瘤醫學研究所 |
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