Rapid purification of lung cancer cells in pleural effusion through spiral microfluidic channels for diagnosis improvement
Journal
Lab on a Chip
Journal Volume
20
Journal Issue
21
Pages
4007-4015
Date Issued
2020
Author(s)
Abstract
Lung cancer is one of the leading causes of death worldwide. Fifteen percent of lung cancer patients will present with malignant pleural effusion initially, and up to 50% will have malignant pleural effusion throughout the course of the disease. In this study, we developed a spiral microfluidic device that can rapidly isolate cancer cells and improve their purity through fluid dynamics. This label-free, high-throughput device continuously isolates cancer cells and other unrelated molecules from pleural effusion. Most of the background cells that affect interpretation are flushed to outlets 1 to 3, and cancer cells are hydrodynamically concentrated to outlet 4, with 90% of lung cancer cells flowing to this outlet. After processing, the purity of cancer cells identified in pleural effusion by CD45 and epithelial cell adhesion molecule (EpCAM) antibodies in flow cytometry will be increased by 6 to 24 times. The microfluidic device presented here has the advantages of rapid processing and low cost, which are conducive to rapid and accurate clinical diagnosis. ? The Royal Society of Chemistry.
SDGs
Other Subjects
Biological organs; Cell adhesion; Diagnosis; Diseases; Fluid dynamics; Fluidic devices; Microfluidics; Molecules; Clinical diagnosis; Epithelial cell adhesion molecules; Lung cancer cells; Micro-fluidic devices; Microfluidic channel; Pleural effusion; Rapid processing; Rapid purification; Cells; epithelial cell adhesion molecule; receptor type tyrosine protein phosphatase C; A-549 cell line; adult; aged; Article; cancer diagnosis; clinical article; controlled study; cytodiagnosis; diagnostic accuracy; female; flow cytometry; human; human cell; immunofluorescence; inflammatory disease; laser microdissection; lung adenocarcinoma; lung cancer; lymphoma; male; malignant pleura effusion; microfluidics; pleura biopsy; pleura mesothelioma; priority journal; sensitivity and specificity; small cell lung cancer; tuberculous pleurisy
Type
journal article