Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences
Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
118
Journal Issue
8
Pages
e2007192118
Date Issued
2021-02-23
Author(s)
Li, Wan Chen
Lee, Chia Yi
Lan, Wei Hsuan
Woo, Tai Ting
Liu, Hou Cheng
Yeh, Hsin Yi
Chang, Hao Yen
Chuang, Yu Chien
Chen, Chiung Ya
Chuang, Chi Ning
Chen, Chia Ling
Hsueh, Yi Ping
Wang, Ting Fang
Abstract
© 2021 National Academy of Sciences. All rights reserved. Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops.
Subjects
Dmc1 | Homologous recombination | Meiosis | Rad51 | Trichoderma
SDGs
Publisher
National Academy of Sciences
Type
journal article