https://scholars.lib.ntu.edu.tw/handle/123456789/552742
標題: | HLA DPB1*0201 allele is negatively associated with immunoglobulin E responsiveness specific for house dust mite allergens in Taiwan | 作者: | CHUNG-YI HU PING-NING HSU R H Lin K H Hsieh K Y Chua |
關鍵字: | Allergen-specific immunoglobulin E; Der p 1; Der p 2; Der p 5; HLA class II genes; House dust mite (HDM); PCR/SSOPH | 公開日期: | 2000 | 卷: | 30 | 期: | 4 | 起(迄)頁: | 538-545 | 來源出版物: | Clinical and Experimental Allergy | 摘要: | Background: House dust mite (HDM) Dermatophagoides pteronyssinus is the most important source of indoor allergens that cause allergic diseases in Taiwan. We prepared purified HDM allergens (Der p 1, Der p 2 and Der p 5) to detect allergen-specific immunoglobulin (Ig) E responsiveness among a large number of test subjects. The robust genetic typing system for HLA class II genes also facilitated the study on association of HLA and allergic response toward HDM. Objective: This study intended to investigate the association between HLA class II alleles and the IgE responsiveness to the major allergens from HDM, D. pteronyssinus. Methods: Two hundred and forty-eight subjects were selected for HLA association study. Plasma HDM allergen (Der p 1, Der p 2, Der p 5) -specific IgE and Der p 2-specific IgG antibodies were detected by ELISA, while HLA class II -DRB1, -DQA1, -DQB1, -DPB1 genetic polymorphism was determined by polymerase chain reaction/sequence-specific oligonucleotide probe hybridization (PCR/SSOPH). Statistical comparison of the allelic distribution of each HLA class II genes among the individuals with/without HDM allergen-specific IgE and IgG antibodies were performed. Results: There was no significant association between HLA DRB1, DQB1, DQA1 alleles and HDM-specific IgE responsiveness noted. Only DRB1*0803 and the linked DQA1*0103 alleles showed positive association with Der p 5-specific IgE responsiveness. However, we found that HLA-DPB1*1301 predisposed subjects to IgE responsiveness to HDM Der p 5. HLA DPB1*0501 was weakly associated with the IgE responsiveness to HDM Der p 1 and Der p 5. There was a strong negative association between the HLA-DPB1*0201 allele with IgE responsiveness to Der p 1 (OR: 0.30, P ? 0.0001, P ? 0.0007, P(c) ? 0.010). Conclusion: We clearly observed the association between HLA DPB1 alleles and specific IgE responsiveness to HDM major allergens. The molecular mechanism of HLA-DPB1*0201 involvement in protecting subjects from HDM- specific IgE responsiveness awaits further investigation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/552742 | ISSN: | 0954-7894 | DOI: | 10.1046/j.1365-2222.2000.00752.x | SDG/關鍵字: | HLA antigen class 2; HLA DP antigen; house dust allergen; immunoglobulin E antibody; immunoglobulin G antibody; allergen; glycoprotein; HLA DP antigen; HLA DPB1; HLA-DPB1; house dust allergen; immunoglobulin E; adult; allele; ambient air; antibody specificity; article; Dermatophagoides pteronyssinus; female; HLA system; house dust allergy; human; major clinical study; male; oligonucleotide probe; priority journal; Taiwan; allele; animal; dust; genetic linkage; genetics; hypersensitivity; immunology; mite; Adult; Alleles; Allergens; Animals; Antigens, Dermatophagoides; Dust; Female; Glycoproteins; HLA-DP Antigens; Humans; Hypersensitivity; Immunoglobulin E; Linkage (Genetics); Male; Mites; Taiwan |
顯示於: | 醫學檢驗暨生物技術學系 |
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