Association of Asef and Cdc42 Expression to Tubular Injury in Diseased Human Kidney
Journal
Journal of Investigative Medicine
Journal Volume
61
Journal Issue
7
Pages
1097-1103
Date Issued
2013
Author(s)
Abstract
Background: Cdc42 is a small guanosine-5′-triphosphatase of the Rho family and plays essential roles in the establishment of cellular polarity and tight junctions in epithelial cells. Adenomatous polyposis coli-associated exchange factor (Asef ) is a canonical guanine nucleotide exchange factor of Cdc42 and renders Cdc42 to be guanosine-5-triphosphate bound and activated. The expression patterns and their significance in human renal diseases are unknown. Methods: We examined the expression of Cdc42 and Asef in kidney biopsy specimens of 15 patients and in normal kidney tissue using immunofluorescence and correlated the expression patterns with the clinical characteristics. We also analyzed the coexpression pattern of Ki-67, a marker indicating cell division, and Asef in selected patients. Results: Expression of Asef and Cdc42 together was associated with tubular injury with 100% specificity. Expression of Asef, regardless of Cdc42, also showed a significant diagnostic odds ratio for the presence of the injury. Expression of Asef was associated with lower estimated glomerular filtration rate at the time of biopsy and larger area of interstitial fibrosis. All Ki-67Yexpressing tubular cells expressed Asef. Conclusions: Induction of Asef and Cdc42 in the renal tubules is a cellular response to injury. Asef induction seems a necessary step for injured tubular cells to enter cell cycle. ? 2013 by The American Federation for Medical Research.
SDGs
Other Subjects
adenomatous polyposis coli associated exchange factor; creatinine; guanine nucleotide exchange factor; Ki 67 antigen; protein Cdc42; unclassified drug; Ki 67 antigen; adolescent; adult; article; cell polarity; clinical article; controlled study; creatinine blood level; disease association; disease severity; female; fibrosing alveolitis; glomerulus filtration rate; human; human tissue; immunofluorescence; kidney biopsy; kidney fibrosis; kidney tubule damage; male; protein expression; sensitivity and specificity; tight junction; aged; Article; diagnostic accuracy; estimated glomerular filtration rate; glomerulus epithelium cell; immunofluorescence test; laboratory test; Adolescent; Adult; Aged, 80 and over; cdc42 GTP-Binding Protein; Female; Gene Expression Regulation; Glomerular Filtration Rate; Humans; Kidney Diseases; Kidney Tubules; Male; Middle Aged; Rho Guanine Nucleotide Exchange Factors; Young Adult
Publisher
BMJ Publishing Group
Type
journal article