|Title:||Activation of human valve interstitial cells by a viridians streptococci modulin induces chemotaxis of mononuclear cells||Authors:||CHIA-TUNG SHUN
|Issue Date:||2009||Journal Volume:||199||Journal Issue:||10||Start page/Pages:||1488-1496||Source:||Journal of Infectious Diseases||Abstract:||
Infective endocarditis is characterized by inflammatory infiltrates of mononuclear cells in infected cardiac valve leaflets. To delineate the role of valve interstitial cells (VICs) in leukocyte recruitment, we stimulated human VICs with glucosyltransferase, a modulin from viridians streptococci. Interstitial cells were activated directly by glucosyltransferase in a dose-dependent manner through concerted mitogen-activated protein kinase and nuclear factor-B signaling pathways; activation resulted in up-regulation of synthesis and release of interleukin-6, interleukin-8, or monocyte chemoattractant protein-1 and enhanced transwell migration ofU937monocytic cells or primary mononuclear cells. The expression of glucosyltransferases and activation of VICs (nuclear localization of RelA) were detected in a rat model of experimental endocarditis. Proinflammatory cytokines also were detected in VICs from diseased human autopsy specimens but not in VICs from normal specimens. These results indicate that interstitial cells in the cardiac valve can be activated directly by bacterial modulins to recruit and retain mononuclear cells, likely contributing to the persistent inflammation characteristic of infective endocarditis. ? 2009 by the Infectious Diseases Society of America.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/553198||ISSN:||0022-1899||DOI:||10.1086/598485||SDG/Keyword:||glucosyltransferase; glucosyltransferase C; immunoglobulin enhancer binding protein; interleukin 6; interleukin 8; mitogen activated protein kinase; monocyte chemotactic protein 1; transcription factor RelA; unclassified drug; alpha hemolytic Streptococcus; animal experiment; animal model; animal tissue; article; autopsy; cell activation; cell migration; cell strain U937; cellular distribution; child; concentration response; controlled study; cytokine production; enzyme binding; experimental model; heart valve; human; human cell; human tissue; male; monocyte; mononuclear cell; neutrophil chemotaxis; nonhuman; priority journal; protein localization; rat; school child; signal transduction; Streptococcus viridans infection; upregulation; Animals; Bacterial Toxins; Cells, Cultured; Chemotaxis, Leukocyte; Child; Disease Models, Animal; Endocarditis; Glucosyltransferases; Heart Defects, Congenital; Heart Transplantation; Heart Valves; Humans; Immunohistochemistry; Leukocytes, Mononuclear; Male; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; RNA, Bacterial; Signal Transduction; Viridans Streptococci
|Appears in Collections:||法醫學科所|
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