https://scholars.lib.ntu.edu.tw/handle/123456789/553206
標題: | Effective prevention and treatment of Helicobacter pylori infection using a combination of catechins and sialic acid in AGS cells and BALB/c mice | 作者: | JYH-CHIN YANG CHIA-TUNG SHUN Chien C.-T. Wang T.-H. |
公開日期: | 2008 | 卷: | 138 | 期: | 11 | 起(迄)頁: | 2084-2090 | 來源出版物: | Journal of Nutrition | 摘要: | The increasing emergence of Helicobacter pylori strains resistant to antibiotics may cause unsuccessful treatment. An alternative agent or mixture with anti-H. pylori effect is urgently required to reduce H. pylori infection. We explored the preventive and therapeutic potential of a combination of catechins and sialic acid on H. pylori-infected human gastric cells in vitro and in mice in vivo. We evaluated the anti-H. pylori activity of catechins and/or sialic acid using the agar dilution and checkerboard methods. The effect of catechins and/or sialic acid on H. pylori infection-induced oxidative stress and apoptosis/autophagy in cell culture was explored using an ultrasensitive chemiluminescence analyzer, immunocytochemistry, and Western blotting. Specific pathogen-free BALB/c mice were divided into uninfected control, infected control, pretreated, and post-treated groups. The effects of catechins/sialic acid were determined by histology and immunocytochemistry. The combination of catechins and sialic acid showed synergistic or additive anti-H. pylori activity and significantly reduced inducible nitric oxide synthase expression and Bax/Bcl-2-mediated apoptosis but enhanced Beclin-1-mediated autophagy. All mice infected with H. pylori displayed gastritis and accumulation of 3-nitrotyrosine and 4-hydroxynonenal. Pretreatment with catechins/sialic acid completely prevented H. pylori infection and resulted in normal histology. Post-treatment with catechins/sialic acid decreased the bacterial load and gastritis score and eradicated up to 60% of H. pylori infections in a dose-dependent manner. This is the first demonstration to our knowledge of a nonprobiotic, nonantibiotic treatment that is 100% effective in preventing and has promising possibilities for treating H. pylori infection. Further studies are needed to confirm this result in humans. ? 2008 American Society for Nutrition. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/553206 | ISSN: | 0022-3166 | DOI: | 10.3945/jn.108.090985 | SDG/關鍵字: | 3 nitrotyrosine; 4 hydroxynonenal; antibiotic agent; catechin; inducible nitric oxide synthase; protein Bax; protein bcl 2; sialic acid; agar dilution; animal cell; animal experiment; animal model; animal tissue; antibiotic resistance; apoptosis; article; autophagy; bacterial strain; cell culture; chemoluminescence; controlled study; disease control; eradication therapy; gastritis; Helicobacter infection; Helicobacter pylori; histopathology; human; human cell; immunocytochemistry; mouse; nonhuman; oxidative stress; Western blotting; Animals; Anti-Bacterial Agents; Catechin; Cell Line, Tumor; Dose-Response Relationship, Drug; Epithelial Cells; Gastric Mucosa; Helicobacter Infections; Helicobacter pylori; Humans; Mice; Mice, Inbred BALB C; N-Acetylneuraminic Acid; Stomach; Stomach Diseases; Bacteria (microorganisms); Helicobacter pylori; Mus |
顯示於: | 法醫學科所 |
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