https://scholars.lib.ntu.edu.tw/handle/123456789/553302
標題: | Differential regulation of interleukin-6 and inducible cyclooxygenase gene expression by cytokines through prostaglandin-dependent and -independent mechanisms in human dental pulp fibroblasts | 作者: | SZE-KWAN LIN YEN-PING KUO Wang J.-S. Lee J.-J. Wang C.-C. Huang S. CHIA-TUNG SHUN Hong C.-Y. |
公開日期: | 2002 | 卷: | 28 | 期: | 3 | 起(迄)頁: | 197-201 | 來源出版物: | Journal of Endodontics | 摘要: | Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-α (TNF-α), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1α or TNF-α and PGE2, either alone or in combination on IL-6 and cyclooxygenase (COX)-2 messenger RNA (mRNA) production in cultured human dental pulp (HDP) fibroblasts. Exposure of HDP fibroblasts to IL-1α or TNF-α resulted in elevated levels of IL-6 (?3.4 to ?10.4-fold) and COX-2 (?5 to ?6.2-fold) mRNA. Simultaneous addition of IL-1α and PGE2 or TNF-α and PGE2 to the cultures significantly reduced the cytokine-induced IL-6 mRNA synthesis ranging from 45% to 65%. However, indomethacin enhanced the cytokine-stimulated IL-6 mRNA synthesis by ?1.7 to ?3.4-fold. This action could be reversed by exogenous PGE 2. In contrast, PGE2 or indomethacin failed to modify the stimulatory effect of IL-1α or TNF-α on COX-2 gene expression. Because excessive levels of IL-6 and prostaglandins have been connected with the pathogenesis of several inflammatory diseases, our results suggest the involvement of HDP fibroblasts in the development of pulpitis via producing IL-6 and COX-2. Furthermore, expression of IL-6 and COX-2 genes in this cell seems to be differentially regulated by cytokines through prostaglandin-dependent and -independent pathways. Copyright ? 2002 by The American Association of Endodontists. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/553302 | ISSN: | 0099-2399 | DOI: | 10.1097/00004770-200203000-00013 | SDG/關鍵字: | cyclooxygenase 2; cyclooxygenase 2 inhibitor; cytokine; indometacin; interleukin 1; interleukin 6; isoenzyme; membrane protein; messenger RNA; nonsteroid antiinflammatory agent; prostaglandin E2; prostaglandin synthase; prostaglandin synthase inhibitor; PTGS2 protein, human; tumor necrosis factor alpha; article; biosynthesis; cell culture; cytology; drug effect; gene expression regulation; human; metabolism; Northern blotting; pulpitis; tooth pulp; Anti-Inflammatory Agents, Non-Steroidal; Blotting, Northern; Cells, Cultured; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors; Cytokines; Dental Pulp; Dinoprostone; Gene Expression Regulation; Humans; Indomethacin; Interleukin-1; Interleukin-6; Isoenzymes; Membrane Proteins; Prostaglandin-Endoperoxide Synthases; Pulpitis; RNA, Messenger; Tumor Necrosis Factor-alpha |
顯示於: | 法醫學科所 |
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